Homework Answers
Answer will be option B
Alex Fluor 488, < 550nm, Alex Fluor 647, >600nm
So Alex 488 allow lower wavelenth of 550nm which means high energy beam can pass which use for excitation purpose while Alex 647 allow greater than 600nm wavelenth light to pass as emission light or beam.
So excitation light should always be high energy and hence low wavelenth while emission should be low energy so high wavelength light.
Hope it's clear..thanks
(i) State which wavelength (primary peak) corresponds to fluorescence and also to phosphorescence...
(i) State which wavelength (primary peak) corresponds to fluorescence and also to phosphorescence? By calculating the energy of each peak, draw the energy level diagram corresponding to the graph below. Highlight transition of light absorbance, fluorescence and phosphorescence emission Briefly state with appropriate diagrams, a) why the phosphorescence emission has a longer lifetime and b) appears at a lower energy with a lower intensity. (ii) (iii) 900 800 700 600 500 400 300 200 100 350 400 450 500 550...
5(15%) Shown below are the absorption and fluorescence spectra for an antibody in pH 72 buffer:...
5(15%) Shown below are the absorption and fluorescence spectra for an antibody in pH 72 buffer: 350 400 450 500 550 600 650 700 750 Wavelength (nm) a. Which is the fluorescence spectrum, the red one or the blue one? Explain. b. What is the best wavelength at which to excite this sample in order to study its fluorescence? Explain. c. What is the best wavelength at which to monitor the fluorescence of this sample for quantitative purposes? Explain. d....
3. Identify the metal ions present in each of the following water sample emission spectra. Use...
3. Identify the metal ions present in each of the following water sample emission spectra. Use the dat in the Table in Question 2 to assist you in identifying the metal ions present in each spectrum. Water Sample 1 672 nm 765 nm and 771 nm 39001 603 nm Intensity 1900 554 nm 900- -100- 400 450 500 700 750 800 550 600 650 Wavelength, nm Identity of metal ions in unknown water sample 1. Water Sample 2 589 nm...
13 14 15 16 17 18 19 2 3 4 5 6 7 8 9 10...
13 14 15 16 17 18 19 2 3 4 5 6 7 8 9 10 If you are using the a sample of 50, must you assume that the population is normally distributed? Yes, since nothing is known about the distribution of the population, ft must be assumed that the population is normally distributed No, because the Central Limit Theorem almost always ensures that is normally distributed when n is large. In this case, the value of n is...
3 4 5 7 8 9 10 11 12 13 14 15 16 17 18 1920...
3 4 5 7 8 9 10 11 12 13 14 15 16 17 18 1920 21 22 23 Question Unit Tutorials If you need help Question 24 O Mark this question Which statement below is NOT true regarding process mapping? O Process mapping forms a foundation for efficiency evaluation O Process mapping is used for evaluating sustainable business solutions. O A process map is a visual representation of the stages in a process. O Almost all business practices and...
12 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17...
12 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Private colleges and universities rely on money contributed by individuals and corporations for their operating expenses. Much of this money is put into a fund called an endowment, and the college spends only the interest earned by the fund. Atrecent survey of 8 private colleges in the United States revealed the following endowments (in millions of 490.0, 122.6, 177.5, 95.4, and 220.0. What...
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16...
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 10kb Probe А 5kb -UI III TIT II Probe B 11kb 7kb — color pink white red pink red pink pink white pink white red pink red white pink pink red pink white pink red pink c. probes A and B: list the animals that show recombination between these markers and then calculate CM distance between them. Which...
C 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16...
C 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 QUESTION 23 10 points Using the toilet is important for people's independence, health and dignity. It can be difficult, however, to teach people to use the toilet when they are aggressive. This study describes how standard toilet training methods can be adapted to help a/3-year-old child with challenging behavior learn to use the toilet independently. Ben was...
6) Which compound would produce this IR spectrum? Wavelength, (um) 3,5 4 4,5 5 5,5 6 6,5 7 8 9 10 12 14 18 2.52,7 3 100...
6) Which compound would produce this IR spectrum? Wavelength, (um) 3,5 4 4,5 5 5,5 6 6,5 7 8 9 10 12 14 18 2.52,7 3 100 80 s 60 40 a 20 0 e 4000 3500 3000 2500 2000 1800 1600 1400 12001000 800 600 400 Wavenumber, (cm-1) (A) (В) CH СОН (C) (D) CH3 СОСН) 7) Which compound is consistent with this IR spectrum? Wavelength, (um) 1003.2 2,4 2.6 3 3,5 4 4,5 5 5,5 6 7 89...
Draw the residual network for the following flow 1/13 15/1 18/29 7/ 0/9 4/6 8/10 0/2 4/4 1/5 0/14 0/4 8/30 7/16 9/2 V3 V8 Draw the residual network for the following flow 1/13 15/1 18/29 7/ 0/9...
Draw the residual network for the following flow 1/13 15/1 18/29 7/ 0/9 4/6 8/10 0/2 4/4 1/5 0/14 0/4 8/30 7/16 9/2 V3 V8
Draw the residual network for the following flow 1/13 15/1 18/29 7/ 0/9 4/6 8/10 0/2 4/4 1/5 0/14 0/4 8/30 7/16 9/2 V3 V8
(i) State which wavelength (primary peak) corresponds to fluorescence and also to phosphorescence? By calculating the energy of each peak, draw the energy level diagram corresponding to the graph below. Highlight transition of light absorbance, fluorescence and phosphorescence emission Briefly state with appropriate diagrams, a) why the phosphorescence emission has a longer lifetime and b) appears at a lower energy with a lower intensity. (ii) (iii) 900 800 700 600 500 400 300 200 100 350 400 450 500 550...
5(15%) Shown below are the absorption and fluorescence spectra for an antibody in pH 72 buffer: 350 400 450 500 550 600 650 700 750 Wavelength (nm) a. Which is the fluorescence spectrum, the red one or the blue one? Explain. b. What is the best wavelength at which to excite this sample in order to study its fluorescence? Explain. c. What is the best wavelength at which to monitor the fluorescence of this sample for quantitative purposes? Explain. d....
3. Identify the metal ions present in each of the following water sample emission spectra. Use the dat in the Table in Question 2 to assist you in identifying the metal ions present in each spectrum. Water Sample 1 672 nm 765 nm and 771 nm 39001 603 nm Intensity 1900 554 nm 900- -100- 400 450 500 700 750 800 550 600 650 Wavelength, nm Identity of metal ions in unknown water sample 1. Water Sample 2 589 nm...
13 14 15 16 17 18 19 2 3 4 5 6 7 8 9 10 If you are using the a sample of 50, must you assume that the population is normally distributed? Yes, since nothing is known about the distribution of the population, ft must be assumed that the population is normally distributed No, because the Central Limit Theorem almost always ensures that is normally distributed when n is large. In this case, the value of n is...
3 4 5 7 8 9 10 11 12 13 14 15 16 17 18 1920 21 22 23 Question Unit Tutorials If you need help Question 24 O Mark this question Which statement below is NOT true regarding process mapping? O Process mapping forms a foundation for efficiency evaluation O Process mapping is used for evaluating sustainable business solutions. O A process map is a visual representation of the stages in a process. O Almost all business practices and...
12 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Private colleges and universities rely on money contributed by individuals and corporations for their operating expenses. Much of this money is put into a fund called an endowment, and the college spends only the interest earned by the fund. Atrecent survey of 8 private colleges in the United States revealed the following endowments (in millions of 490.0, 122.6, 177.5, 95.4, and 220.0. What...
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 10kb Probe А 5kb -UI III TIT II Probe B 11kb 7kb — color pink white red pink red pink pink white pink white red pink red white pink pink red pink white pink red pink c. probes A and B: list the animals that show recombination between these markers and then calculate CM distance between them. Which...
C 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 QUESTION 23 10 points Using the toilet is important for people's independence, health and dignity. It can be difficult, however, to teach people to use the toilet when they are aggressive. This study describes how standard toilet training methods can be adapted to help a/3-year-old child with challenging behavior learn to use the toilet independently. Ben was...
6) Which compound would produce this IR spectrum? Wavelength, (um) 3,5 4 4,5 5 5,5 6 6,5 7 8 9 10 12 14 18 2.52,7 3 100 80 s 60 40 a 20 0 e 4000 3500 3000 2500 2000 1800 1600 1400 12001000 800 600 400 Wavenumber, (cm-1) (A) (В) CH СОН (C) (D) CH3 СОСН) 7) Which compound is consistent with this IR spectrum? Wavelength, (um) 1003.2 2,4 2.6 3 3,5 4 4,5 5 5,5 6 7 89...
Draw the residual network for the following flow 1/13 15/1 18/29 7/ 0/9 4/6 8/10 0/2 4/4 1/5 0/14 0/4 8/30 7/16 9/2 V3 V8
Draw the residual network for the following flow 1/13 15/1 18/29 7/ 0/9 4/6 8/10 0/2 4/4 1/5 0/14 0/4 8/30 7/16 9/2 V3 V8
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