Question

EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of position, and place a drop of immersion oil on the area of the slide you are observing. Carefully click the oil immersion lens into position. It should now be immersed in the oil (FIGURE 1.5). Careful use of the fine-adjustment knob should bring the object into focus. Note the shape and size of the cells. Did the color of the cells change with the different lenses?(5). — Did the size of the field change? (6). - 10 Record your observations, and note the magnifications. (Use the following figures as references: Algae: Figure 2.2a and b on page 21 and 34.3 on page 273: protozoa: Figure 2.2c on page 21 and 35.1 on page 279; fungi: Figure 33.2 on page 262) 11. Repeat this procedure with all the available slides. When observing the bacteria, note the three different morphologies, or shapes, shown in FIGUR E 1.6 on the next page. When your observations are completed, move the turret to bring a low-power objective into position. Do not rotate the high-dry (40X) objective through the immersion oil. Remove the slide. Clean the oil off the objective lens with lens paper, and clean off the slide with tissue paper or a paper towel. (a) Move the high-dry lens out of position. (b)Place a drop of Immersion oil in the center of the slide. (e) Move the oil immersion lens into position FIGURE 15 Using theoilimmersion lens.

Answer 1

5. The colour of the cells does not change with the magnification power of objective lenses. Magnification results in the change in the size of cells and has no effect on the colour of the cell. The colour is determined by the stain which is used to stain the cells before observing them under the microscope.

6. The size of the field changes with the magnification power of objective lenses. As the magnifying power of lens is increased, the area of field decreases and vice versa.

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