Question

EXERCISE 1:USE AND CARE OF THE MICROS D CARE OF THE MICROSCOPE 11. Alter use, remove the slide, wipe oil of it, put dist cover on the microscope, and return it to the designated area. 12. When a problem does with the microscope obtain help from the instructor. Do not use other microscope unless yours is declared out of action." FIGURE 14 Focusing the condenser. () Ching low power, lower the condenser til distinct circle of light is visible. Cester the code of light wing the string Open the inis diapliran until the lightjust fille MATERIALS Compound light microscope Immersion oil Lens paper and optical lens cleaner Prepared slides of algae, fungi. protozon, and bacteria PROCEDURE 1. Place the microscope on the bench squarely in front of you. 2 Obtain a slide of algae, fungi, or protozon, and place it in the clips on the mechanical stage. 3 Adjust the eyepieces on a binocular microscope to your own personal measurements. a. Look through the eyepieces and using the thumb wheel adjust the distance between the eyepieces until one circle of light appears. b. With the low-power (OX) objective in place, cover the left eyepiece with a small card and focus the microscope on the slide. When the right eyepiece has been focused, remove your hand from the focusing knobs and cover the right eyepiece. Looking through the micro- scope with your left eye, focus the left eyepiece by turning the eyepiece adjustment. Make a note of the number at which you focused the left eyepiece so you can adjust any binocular microscope for your eyes. 4 Raise the condenser up to the stage. On some microscopes, you can focus the condenser by the following procedure: a. Focus with the loX objective. b. Close the iris diaphragm so only a minimum of light enters the objective lens. c. Lower the condenser until you see the light as a circle in the center of the field. On some microscopes, you can enter the circle of light (FIGURE 14) by using the centering screws found on the condenser d. Raise the condenser up to the slide, lower it, and stop when the color on the periphery changes from pink to blue (usually lor 2 mm below the stage). 5 Open the iris diaphragm until the light just fills the field. Adust the contrast by changing the diaphragm opening Diagram some of the cells on the slide under low power. Use a minimum of light by adjusting the 7. When you have brought an image into focus with low power, rotate the turret to the next lens, and the subject will remain almost in focus. All of the objectives (with the possible exception of the 4X) are parafocal: that is, when a subject is in focus with one lens, it will be in focus with all of the lenses. However, the working distance that is, the distance between the objective lens and the specimen-will change (Figure L2). In general, the working distance decreases as magnification increases. When you have completed your observations under low power, swing the high-dry objective into position and focus. Use the fine adjustment. Only a slight adjustment should be required. Why? (1) More light is usually required at higher magnifica- tion. How can you increase the amount of light?(2) 3. Again, draw the general size and shape of some cells. A & B (3-4). Add name, magnification and details.

Answer 1

ANSWER 7 :-

1. The lens used is a parfocal objective lens meaning that if one objective lens is focussing the specimen, then the remaining lenses also will focus the specimen. So, even at the time of using the 40X objective lens, there will be decrease in distance between the specimen and the objective although the adjustment should be fine as during the utilisation of the 10X lens, the image was still in focus and as said earlier the image will still remain in focus for the high-dry /objective and hence only minor adjustments are required.

ANSWER 8:-

1. The microscope is equipped with a structure called as Diaphragm. The diaphragm is placed below the stage onto which the specimen is placed for study.
2. Basically a light source is being directed towards a condensor which is associated with consensation of light arising from a souce which is then directed towards the aperture or the diaphragm which controls the amount of light being projected onto the stage. This can be done by means of rotation of the diaphragm.

NOTE :- Respected Sir/Madam, for any doubts, please prefer communicating through the comment section and please provide an upvote if the answer seems satisfactory. There is no image regarding the type of cells being studied and hence answer 9 cannot be stated.