Question

please please help with all of them no thorough explanation needed!

5. Select the most appropriate answer. When using a dichotomous key outside to identify unknown organisms one should

A. not remove parts of the plant or disrupt the ecosystem

B. be aware of hazards such as ticks and poison ivy

C. should write down the steps followed on the key.

D. Be aware that your best attempts to identify a specimen might be disrupted by the presence of sexual dimorphism or polymorphism, juvenile, and adult phase.

E. all of the above are important things to consider

6. How would the resolving power (resolution) change if one were to use ultra violet light instead of visible light in a microscope?

A. the value would decrease giving poorer resolution

B. the value would increase giving better resolution.

C. the value would decrease giving better resolution.

D. it would not change the resolution

7. Identify when negative stains are primarily used. Note, more than one option may be correct.

A. to view endospores

B. to stain cells that are too delicate to be heat-fixed

C. where accurate size determination is crucial.

D. To differentiate between Gram positive and Gram negative organisms.

8. Assume that the slide and all lenses are clean and the image is in SHARP focus. To obtain maximum resolution on the microscope what part would you adjust (without changing the objective)?

A. fine focus

B. iris diaphragm

C. diopter ring

D. course focus

9. Select which of the following statements is NOT correct.

A. Lowering the condenser decreases the amount of light that will reach the specimen.

B. oil should only be used with the 100X objective

C. Resolution is dependant on the wavelength of light and the numerical aperture of the lens system

D. resolution is improved by an increase in numerical aperture and an increase in the wavelength of light

10. The oil immersion or 100x objective lens provides the highest magnification.

a. True

b. False

11. Calculate the resolving power of a microscope with the numerical aperture of 1.25. The wavelength of visible light is 560 nm.

A. .5 nm

B. 0.2 um

C. 0.2 nm

D. 0.4 um

12. The most useful adjustment for increasing image contrast in low-power magnification is

A. using a blue filter.

B. adjusting the aperture iris diaphragm.

C. closing one eye.

D. placing a drop of oil on the slide.

13. The oil- immersion or 100x objective lens has the shortest working distance.

A. True

B. False

14. You have been asked by your boss to buy a compound microscope. You view two microscopes priced the same but with different numerical aperture values. Which will you purchase, a microscope with a higher or lower numerical aperture?

A. higher

B. lower

C. both are equal

Answer 1

As this is having more the four question I am answering five of them

Q How would the resolving power (resolution) change if one were to use ultra violet light instead of visible light in a microscope?

Ans: B-the value would increase giving better resolution because the resolution is dependent on the wavelength, and it is inversely proportional  i.e., higher wavelength would give lesser resolution

Q: You have been asked by your boss to buy a compound microscope. You view two microscopes priced the same but with different numerical aperture values. Which will you purchase, a microscope with a higher or lower numerical aperture?

Answer: A. higher; higher numerical aperture allows more light to lens thus offers better resolution

Q: The oil- immersion or 100x objective lens has the shortest working distance.

Answer. A-True; because the distance between lens and element is very less

Q: The oil immersion or 100x objective lens provides the highest magnification.

Answer a. True, the name it self indicate (100X; or 100 times)

Q: Identify when negative stains are primarily used. Note, more than one option may be correct.

Answer: it is used for multiple purpose such as (B). to stain cells that are too delicate to be heat-fixed (C). where accurate size determination is crucial.