Question

Evaluate the following statements, and select those that correctly apply to the properties of microscopes. When observing specimens with the 100x lens, adding a drop of oil to the slide will improve the resolving power; the image will be clear rather than blurry. If oil is not used with the 100x lens, the virtual image will be less bright, compared to specimens observed with a drop of oil on the slide. The degree of contrast between a specimen and its surroundings is enhanced by the use of special stains and opening the iris diaphragm wide to allow in maximum light. Light rays with shorter wavelengths enhance the ability to resolve objects that are close together. If the 40x high-powered lens is used to view a specimen in conjunction with a 10X ocular, the total magnification of the specimen will be 50x. Reset

Answer 1

When observing specimens with the 100x lens, adding a drop of oil to the slide will improve the resolving power; the image will be clear rather than blurry. True. In fact, the oil is called immersion oil - and it is used to observe organelles, defined structures and bacteria under 100X If oil is not used with the 100x lens, the virtual image will be less bright, compared to specimens observed with a drop of oil on the slide. The degree of contrast between a specimen and its surroundings is enhanced by the use of special stains and opening the iris diaphragm wide to allow in maximum light. False. Narrower widths of the iris diaphragm produce more contrast → True. The shorter wavelength, the greater resolution Light rays with shorter wavelengths enhance the ability to resolve objects that are close together. → False. The total magnification would be 40X x 10X = 400X If the 40x high-powered lens is used to view a specimen in conjunction with a 10X ocular, the total magnification of the specimen will be 50x.