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ur eyes. Each new lab day, as you begin to study your arst slide, repeat this procedure, arssm increase or decrease the contrast by adjusting the iris dia- u em opening. Note that the maximum amount of light provides itle contrast. Adjust the aperture until the imageis sharp. littie Move the slide slowly to the right. In what direction does the image in the ocular move? n. Is the image in the ocular inverted relative to the specimen on the stage? Center the specimen in the field of view; then rotate the 40x objective o. into position while watching from the side. Ifit appears that the objective will hit the slide, stop and ask for assistance. Most of the microscopes have parfocal lenses, which means that little refocusing is required when moving from one lens to another. If your scope is not parfocal, ask your instructor for assistance. After the 40x objective is in place, focus using the fine adjustment knob. P. Never focus with the coarse adjustment knob when you are using the high-power objective. The distance between the specimen and the objective lens is called the working distance. Is this distance greater with the 40x or the 10X objective? q. 3. Compute the total magnification of the specimen being viewed. To do so, multiply the magnification of the ocular lens by that of the objective lens. What is the total magnification of the letter as the microscope is now set? a.
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