Question

QUESTION 6 When viewing lide, which should you begin with? (Pick the best answer) QUESTION 7 What part of the Inverted microscope is different than on a brightfield microscope? The coarse adjustment knob All of these The objective lens magnification, The fine adjustment knob The light path The stage QUESTIONS When switching from the high-dry objective to the oil immersion lens on a light microscope, which of the following steps would you NOT perform? Placing a drop of immersion oil on the slide Centering the object in the field of view before changing objectives All of these ARE steps involved with using the oil immersion lens Using the large coarse focus knob to improve image quality Adjusting the light intensity and contrast to appropriate levels

Answer 1

6. 4X objective lens is used for viewing the image for the first time because it has the lowest resolution power and thus has the highest field of view. This helps the observer to locate the specimen on the slide and further go on to higher resolution objective lens.

7. The light path is different in brightfield microscope from inverted microscope. In inverted microscope, the light sourse is above the stage and objective below the specimen and stage whereas in brightfield microscopes, the light souce and objective is beneath the stage.

8. Using large course focus knob to improve image quality is strictly prohibited while switching from high dry objective to oil immersion lens as you can damage the lens. Only fine adjustment knob is used.