Question

1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could LM be used? If so, name one type/technique Could EM be used? If so, what kind of EM and any specific staining/preparation? Movement of chromosomes during anaphase The distribution of membrane proteins that span the lipid bilayer of the plasma membrane in plant cells Cell viability (determining if cells are alive?) Cristae in a plant cell mitochondrion Determining if 2 specific proteins are interacting with each other in a living animal cell

Answer 1

Movement of chromosome during anaphase	Yes.in standard epi-fluorescence microscopy technique.	Yes. Scanning electron microscopy
The distribution of membrane proteins that span the lipid bilayer of the plasma membrane of plant cell	No.	Yes.in freeze-fracture electron microscopy(Transmission Electron Microscope)
Cell viability(determining if cells are alive?)	Yes.in fluorescent microscope with DAPI (blue) and FITC (green) filter. NucBlue (stains live cells) and NucGreen (stains cells with compromised cell membrane/ dead cells)	Yes.scanning electron microscopy.
Cristae in a plant cell mitochondrion.	No.	Yes.Transmission electron microscopy
Determining if 2 specific proteins are interacting with each other in a living animal cell	Yes.Fluorescence microscopy	Yes.transmission electron microscope