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5. You are looking at a specimen that, being stained with eosin and hematoxylin looks primarily red. What color filter would you use to increase the contrast of the image?

6. If you are looking at a specimen and put a single polarizing filter between the light source and the specimen have you improved the resolution of the image? Have you improved the contrast of the image? 7. If you observe a specimen under crossed polarizers and see something shining brightly, what does this tell you about the brightly shining structure? What is this phenomenon called? 8. You aregiven a slide of living unctained cells. What adjustments would you make to your microccope to encure that you were able to best obcerve the cells? 9. What are some of the ways in which you can increase the contrast of your microccope?You chould concider adjustments that can be made with the condenser, iris diaphragm various filters, etc. 10. What adjustments might you make to increase the resolution of your microscope? Consider adjustments to the condenser, iris diaphragm various filters, etc.

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5. Didymium filters will be used . It is also known as enhancing filter . It is made of didymium glaas to increase the intensity and saturation of red objects. Thin section of biological tissues are stained with one or more dyes to enhance visiblity of specimen , if taking photo of these specimen it is desirable for the stained colour to appear in the photo . While most stain show well on coloured film , there are several that appear washed out , this didymium filter help to combat this washed out problem.

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