Question

1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy [EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could LM be used? so, name one type/technique Could EM be used? If so, what kind of EM and any specific staining/preparation? Movement of chromosomes during anaphase The distribution of membrane proteins that span the lipid bilayer of the plasma membrane in plant cells Cell viability (determining if cells are alive?) Cristae in a plant cell mitochondrion Determining if 2 specific proteins are interacting with each other in a living animal cell . Briefly state the function of each of the following parts of a light microscope. a) Field iris diaphragm: Controls FOCOUS b) Condenser iris diaphragm: controls the amount of the reaching the Speed c) Condenser focus knob: MOSCOhouse to control a crocion which is sccused to prodeo

Answer 1

Structure Or Phenomenon	For LM	For EM
movement of chromosomes during anaphase	Fluorescence Microscopy	no
the distribution of membrane protein that span the lipid bilayer of the plasma membrane in plants	No	transmission electron microscopy
Cell Viability (Cells are dead or alive )	Compound microscope	these microscopes cannot be used to image living cells because the electrons destroy the samples
cristae in plant cell mitochondria	Fluorescence microscopy	No
determining if 2 specific proteins are interacting with each other in a living animal cell	fluorescence microscopy	No

a) Iris Diaphragm controls the amount of light reaching the specimen

b) Condenser is used to collect and focus the light from the illuminator on to the specimen. It is located under the stage often in conjunction with an iris diaphragm

c) Condenser focus Knob =This control is used to precisely adjust the vertical height of the condenser