Question

69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue

70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat

71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane

(b) the periplasmic space (c) teichoic acids (d) lipid A (e) gram positive cell walls

72. WATER is used in most staining to... (a) dilute the dye to proper proportions (b) remove excess (c) dilute the smear (d) remove all the primary dye (e) none of these

73. Officially, ACID FAST vegetative cells stain _?_ on the Gram stain. (a) positive (b) negative (c) Gram variable on the positive side (d) none of these is correct

74. Endospores are always.. (a) small & roundish or oval (b)egg-shaped (c) rod shaped (d) often appear "clear&smaller than the vegetative cels that "made" them (e) all of these could be correct except letter

75. THE ENDOSPORE itself is supposed to/should... stain a_?_ color in an Endospore POSITIVE STAIN. (a) HOT pink (b) pink (c) purple (d) green (e) baby-blue

76. A staining procedure that allows the differentiation of the genus Nocardia is the_? stain. (a) Negative (b) Acid Fast (c) Gram (d) Endospore (e) Flagella

77. THE MAJOR stain in MICROBIOLOGY is known as the_? stain. (a) Negative (b) Acid Fast (c)Gram (d) Endospore (e) Flagella

78. TEICHOIC ACIDS are found: (a) in Gram positive microbes (b) in Acid Fast microbes (c) as a part of cell wall (d) on the cell wall as parasitic attachment sites (e) all of these are true except answer "b"

79. The Gram negative bacteria have _?_ cell membrane(s). (a) 2 (b) 1 (c) 0 (d)3 (e) 5

80. Which of the part of some microbes is responsible for causing fever, blood vessel dilation and possibly SHOCK? (a) the plasma membrane (b) the periplasmic space (c) teichoic acids (d) lipid A (e) gram positive cell walls

81. What do Endospore positive microbes stain on the Gram stain? (a) purple (b) blue (c) "hot" pink (d)pink (e) green

82. What do Endospore positive microbes stain on the Acid Fast stain? (a) purple (b) blue (c) "hot" pink (d)pink (e) green

83. Which of the following is NOT true concernina ENDOSPORES? (a) inactive metabolically (b)Chemically resistant (c) heat and radiation resistant (d) a survival structure (e) helpful reproduction

84. The GRAM stain has two pigmented dyes and a total of four solutions. What is the secondary dye called? (a) crystal Violet (b) Safranin (c) Acetone Ethanol (d) Gram's lodine Solution (e) none of these is correct

85. Cells from the MYCOBACTERIUM GENUS usually stain a __?__ color on the Gram Stain. (a) “hot” pink (b) pinkish (c) purple (d) green (e) baby-blue

86. Gram POSITIVE cells stain _?_ in color. (a) "hot" pink (b) pinkish red (c) purple (d) green (e) baby-blue

87. Non-acid Fast cells stain _?_ in color. (a) "hot pink" (b) pinkish red (c) purple (d) green (e) baby-blue

88. Acid Fast cells stain_?_ in color. (a) fuchsia (b) pink (c) purple (d) green (e) baby-blue

89. ENDOSPORE negative cells stain ? in color. (a) fuchsia (b) pink (c) purple (d) green (e) baby-blue

90. What do Gram negative microbes stain on the Acid Fast stain? (a) purple (b) blue (c) "hot" pink (d) pink (e)green

91. The PRIMARY dye in the Gram stain is known as: (a) methylene blue (b) iodine solution (c) safranin

(d) ethanol (e) crystal violet

92. You have made a stained prep. You are beginning to view the slide. You place the slide on the stage in the "cut-out"' with oil and turn on the light. You move the slide "circle" over the hole in the stage. You rotate the nosepiece ring from the 4x to the 10x objective and set the eyepiece width correctly. You bring the stage all the way up. You look through the eyepiece but the field is yellow and dark. What is the MOST common error in focusing the microscope? (a) your light power is low (b) your condenser is down (c) your diaphragm is closed (d) your objective is not "clicked' into place (e) none of these is incorrect

93. What do Acid Fast microbes stain on the Gram stain? (a) purple (b) blue (c) "hot" pink (d) pink (e)green

94. Which of the following is NOT part of the Condenser apparatus? (a) filter (b) condenser lens (c) iris (d)light source (e) none of these is incorrect

95. When a properly stained, properly focused slide of bacteria appears to "flow like a river" under the 100X objective, what is your most likely error? (a) a failure to heat fix properly (b) over washing (c) using too much or too little immersion oil (e) using too much alcohol or letting it stay on the smear prep too long

96. When should you apply immersion oil to the smear? (a) after blotting (b) after focusing under 10x (c) just before rotating the 100x objective into place (d)directly after heat fixing (e)none of these is exactly correct

97. The reason we use type A oil immersion oil is because this oil and the glass in our scopes and slides have the same index of_?_ (a) reflection (b) refraction (c) resolution (d) diffraction (e)luminescence

98. The _?_ controls the amount of light that passes directly through our specimen and into the objective lens. (a) nosepiece (b) ocular (c) condenser lens (d) iris diaphragm (e) arm

99. Which of the following is a part of the eyepiece? (a) micrometer (b) pointer (c) eye-width adjustment on binocular scopes (d) 10Ox power (e) none of these is incorrect

Answer 1

69. D)Green

Endospores will be stained green using malachite green and it will look green in colour

70. C) smear

Preparing a perfect smear is important in staining procedure

71. d) lipid A

Lipid A is the endotoxin that is responsible for septic shock

72. B) remove excess dye

As per policy, it is allowed to answer first 4 questions.