Question

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21- Which of the following is an acid fast microorganism a) E. col b) S aureus c) S pneumonia d) Mycobacterium smegmatis 22-23 You are given a mixed culture containing E: coli and M. luterus. Explain how you would isolate them and prepare a pure culture of each. Be specific. is ideal due to 24-To cultivate fungi, a selective medium such as its low pH that inhibits bacterial growth. a. Sabouraud agar b. Nutrient agar c. Mueller Hinton II d. None of the above 25-Molds have microscopic intertwining filaments called a. Hyphae b. Septae c. Basidiomycetes d. Ascomycetes 26- Special stains in Microbiology include a. Spore stain b. Flagella stain c. Gram stain d. Acid fast stain e. Both a and b 27- A method of spore staining that stains the endospore green and the vegetative cell is the a. Dorner method b. Anthony method c. Schaeffer-Fulton d. santos method

Answer 1

21. Mycobacterium smegmatis is an acid-fast bacterial species of phylum Actinobacteria an genus Mycobacterium. With a bacillus shape long and stained by ziehl-neelsen method.

22-23 . Streak plate method of isolation is most common way of separating bacterial cells on agar surface to obtain isolated colonies. The purpose of streak plating is isolate individual bacterial cells on a nutrient medium.

24. To cultivate fungi a selective medium such as Sabouraud agar is ideal due to its pH which inhibits bacterial growth.Sabouraudagar is a type of medium containing peptones, which is used to cultivate dermatophytes and other types of fungi, The acidic PH (5.6) of sabouraud agar inhibits bacterial growth.

25.Molds have microscopic intertwining filaments called Hyphae. because mold is fungus with multicullar filaments which are called Hyphae.

26. special stains in microbiology are both a and b like spore stain and flagella stain because special stains provide valuable information in the evaluation of numerous abnormal or diseases.

27.Schaeffer. Fulton method which is used to distinguish between the vegetative cells and the endospores.