Question

NEED HELP WITH ALL PLEASE. MICROBIOLOGY

You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple cocci B. Purple rods C. Pink cocci D. Pink rods E. Fuchsia (RED) rods 4. Your Pseudomonas aeruginosa sample when viewed on the slide is all purple what might have gone wrong in the staining. A Nothing the organism is Gram positive and should be purple B. Too much Crystal Violet was used. C. Not enough ethyl alcohol was used D. Too much ethyl alcohol was used

Answer 1

1.) ® Green and it stained ( Endosbove are stained with Malachite green green in colour). ?) A Colourless (Primary stain will be washed out by alcohol leave cell stained * ® Purplebotomy essa) (Bli cheniformis commonly found in soil is arod shaped, gram positive bacteria gire purple colour due to Crystal riolet staining) © © Not enough ethyl alcohol was used. Cif P. aaruginosa sive purple colour instead of pink than primary stams does not washed out by alcohol property 6 False. CEthyl alcohol step is not about timing beet about time washed until briman staim wash out but not for long tham cell. Loall will dissolve.) 6 True (Grom staining is all about cell wall composition that vam in both types of bacteria sive different staina) Has advised to wash with water after alcohol wash to leber wash with excess alcohol & ② lodine Clodine is always used as mordant) O E Crystal violet Crystal widlet is used as primamstain (10 Estry) alcohol (ethyl alcohol used to de colorire brimam stains). а) - True.