Question

Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the cells below. Show the steps in Gram staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Gram Staining for G+ and G cells. A. Gram Positive B. Gram Negative Step 1: Add Crystal Violet Step 1: Add Crystal Violet Step 2: Add_ Step 2: Add (make sure you show what happens to the reagents when this is added) Step 3: Add Step 3: Add (make sure you show what happens to the reagents when this is added) Oʻololo Step 4: Add Step 4: Add End: Color of cell End: Color of cell

Answer 1

Gram staining : - ram skaining or gram's method is differential staining memod for differentiating baclesia spicies into two groups based on the physical propenies of their collwalls Discover by Danish scientist Hans Christian Grom in 1884. Trice to remb. CIDS sofranin Crystal Toler Iodine be colonization (Allohd wash) → Iodine act as mordant you increase the finiry of crystal violet - Decatrizer for removing sain , which not retain by cell well. - safranin - act as counter slain. Add Ingstal violet Depi Add Isral vide- Step 1- < A WOOD after adding Crystal vister, impan purple color to all well Step-as Add rodine Solution o Blepa - Add Iodine solut 000 After adding iodine, ffinity of crystal stair increase Step . Adbl decolorizers Step 3. Add decebrizer Akter adding decolon by decolo h2o1. , Stein which not 4 чаһон хаіл on аll uz, моп

Step 4 - Add sofranin a Stepe had sapanino After adding safran in the baller which relain Crystal violet Stain semain purple and the bacteria which not retain systel violet look colouness but ofter sapronin addition they appear pinkor redi from positive purple at mencan Grom Negative Pink pey