A common first step in staining procedures such as the Gram stain is to prepare a...
A common first step in staining procedures such as the Gram stain is to prepare a heat-fixed bacterial smear. Why is this technique NOT the best for accurately detemining the size of the bacterial cell?
a. the heat will prevent ethanol from dissolving the inner membrane and makes it hard to decipher cell size
b. the heat will enhance the formation of crystal violet-iodine complexes, which will make the bacteria appear larger than they actually are
c. the heat fixes bacteria to the glass slide, but also causes the cells to shrink
d. Both A and C
e. All of the above
Homework Answers
Option c is correct. the heat fixes bacteria to the glass slide, but also causes the cells to shrink.
Add Answer to:
A common first step in staining procedures such as the Gram
stain is to prepare a...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order,...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media)...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells,...
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells, a small halo will be seen around the cells. Why is this the case? What external structure of Gram-negative cell will acude the negative stain? 2 What are the functions of a bacterial capsule? 3 Does a species produce a capsule under all conditions of cultivation? Explain. 4 Why is the size of a bacterium more accurate for a negative stain than a direct...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
adım sua LISNA SPECIAL MATCHING Match only within the horizontal lines. Match the items on the...
adım sua LISNA SPECIAL MATCHING Match only within the horizontal lines. Match the items on the right with the termsstatements questions on the left to give the one best answer. Use answers only once. Use all. 54. White colonies, G-) rods in chains 2. Bacitus 55. Pink colonies, G-) rods, irregular b. Mycobacterium 56. Yellow colonies, G(+) cocci in tetrads C. E. coli 57. Endospore forming, G(+) rods in chains d. Serratia 58. Acid fast, G(+) rods, irregular Micrococcus luteus...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells, a small halo will be seen around the cells. Why is this the case? What external structure of Gram-negative cell will acude the negative stain? 2 What are the functions of a bacterial capsule? 3 Does a species produce a capsule under all conditions of cultivation? Explain. 4 Why is the size of a bacterium more accurate for a negative stain than a direct...
adım sua LISNA SPECIAL MATCHING Match only within the horizontal lines. Match the items on the right with the termsstatements questions on the left to give the one best answer. Use answers only once. Use all. 54. White colonies, G-) rods in chains 2. Bacitus 55. Pink colonies, G-) rods, irregular b. Mycobacterium 56. Yellow colonies, G(+) cocci in tetrads C. E. coli 57. Endospore forming, G(+) rods in chains d. Serratia 58. Acid fast, G(+) rods, irregular Micrococcus luteus...
Most questions answered within 3 hours.
-
The blues made its way into many kinds of music. Eric Clapton,
The Beatles, and Elvis...
asked 1 hour ago -
8. A wave in a string has a wave function given by: y (x, t) =...
asked 1 hour ago -
If you’re standing at the bottom of a hill and asked to evaluate
it while being...
asked 2 hours ago -
1. Which region has taken the lead in the world of
e-waste handling?
a) European Union...
asked 2 hours ago -
A 8.15- g bullet from a 9-mm pistol has a velocity of 366.0 m/s.
It strikes...
asked 4 hours ago -
The outstanding bonds of Alpha Extracts have a yield to maturity
of 7.4 percent and a...
asked 4 hours ago -
The Problem: The Case of the Harmonizing Vacations
Your CEO is exploring partnering with a European...
asked 5 hours ago -
A chemical equation is balanced by adding coefficients in front
of some formulas so that the...
asked 5 hours ago -
From the literature (reference your sources): What are the
lattice parameters of calcite and aragonite? Why...
asked 6 hours ago -
Your system is rejecting the question am asking which is
preceded by a case study. It...
asked 6 hours ago -
3. On January 2, 2000, Larry creates a trust with himself as
trustee. Larry as trustee...
asked 6 hours ago -
A member of the volleyball team spikes the ball. During this
process, she changes the velocity...
asked 6 hours ago