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5. Why are simple stains able to stick” to bacteria? Explain the chemical properties of the stain and bacteria surface.
3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Asep
affect the actual size of the bacteria by causing it to shrink or distort slightly. All of these effects may be potential rea
Glass slides Inoculating loop Bunsen burner Bibulous paper Sharpie or wax pencil Bacterial cultures (broth or solid media) Ba
4. After the slide has air dried completely, it must be heat fixed. Heat fixation kills the bacteria and prevents them from b
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Answer #1

This is because of opposite charges of simple stain and bacterial cell surface.

Bacterial cell walls are negatively charged and simple stains are positively charged. Stain molecules are entrapped between the meshwork of cell wall of bacteria and remain associated there due to interaction between positively charged stain molecules and negatively charged molecules present in the cell wall of bacteria.

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