Question

Acid Fast Staining Carbolfuchsin Acid-Alcohol Q Methylene Blue Using the reagents above and the cells below. Show the steps in Acid Fast staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Acid Fast Staining for Acid Fast+ and Acid Fast-cells. A. Acid Fast Positive Step 1. Add Carbolfuchsin B. Acid Fast Negative Step 1. Add Carbolfuchsin Step 2. Add Step 2. Add (make sure you show what happens to the reagents when this is added) olo o Step 3. Add Step 3. Add (make sure you show what happens to the reagents when this is added) End: Color of Cell End: Color of Cell

Answer 1

The characteristic difference between acid fast bacteria and other organisms is the presence of a thick, waxy wall (made up of mycolic acid) that makes penetration by stains very difficult. Once the stain has penetrated, however it cannot be readily removed even with the vigorous use of acid alcohol as the decolorizing agent, because of this property these organisms are called acid fast, while all other microorganisms which can be easily decolorized by acid alcohol are called as non-acid fast.

END RESULT:

ACID FAST BACTERIA WILL BE RED IN COLOUR

NON-ACID FAST BACTERIA WILL BE BLUE IN COLOUR

Acid Fast Staining Carbolfuchsin Acid-Alcohol Q Methylene Blue Using the reagents above and the cells below. Show the steps in Acid Fast staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Acid Fast Staining for Acid Fast+ and Acid Fast-cells. A. Acid Fast Positive Step 1. Add Carbolfuchsin B. Acid Fast Negative Step 1. Add Carbolfuchsin Step 2. Add Acid-Alcohol Step 2. Add Acid-Alcohol (make sure you show what happens to the reagents when this is added) Acid-Alcohol Methylene Blue Step 3. Add Step 3. Add Methylene Blue (make sure you show what happens to the reagents when this is added) End: Color of Cell BLUE COLOUR End: Color of Cell RED COLOUR