You have 2 unknown Bacteria, You need to find out what these bacteria are there biochemical test are:
Unknown #1---------------------------
There is a chart on page 3 that can help you.
Test |
Initial Color |
Final Color |
Result |
Lactose |
Red |
Yellow |
with gas |
Sucrose |
Red |
Red |
with no gas |
Simmons Citrate |
Green |
Green |
Negative |
urea |
Peach |
Peach |
Negative |
SIM |
Yellow |
S = - I = + M = + |
|
Gram Stain & Shape |
Red |
Gram - & Rod |
Unknown #2 ---------------------------
There is a chart on page 3 that can help you.
Test |
Initial Color |
Final Color |
Result |
Lactose |
Red |
Yellow |
with no gas |
Sucrose |
Red |
Yellow |
with no gas |
Simmons Citrate |
Green |
Blue |
positive |
Urea |
Peach |
pink |
positive |
SIM |
Yellow |
Yellow |
S = - I = - M= - |
Gram Stain & Shape |
Violet |
Gram+ , Cocci |
Test |
E.coli |
Enterobacter aerogenes |
Staphylococcus aureus |
Streptococcus pyogene |
|
Lacrose |
Acid/Gas |
Acid/No Gas |
Acid/No Gas |
Acid |
|
Sucrose |
No Acid/No Gas |
Acid/No Gas |
Acid/No Gas |
Acid |
|
Urea |
Negative |
Negative |
Positive |
Negative |
|
Indole |
Positive |
Negative |
Negative |
Negative |
|
Citrate |
Negative |
Positive |
Positive |
Negative |
|
H2S |
Negative |
Negative |
Negative |
Negative |
|
Motility |
Positive |
Positive |
Negative |
Non-Motile |
|
Shape |
Rod |
Rod |
Cocci |
Cocci |
|
Gram Stain |
G - |
G- |
G + |
G+ |
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