Question

Paragraph Styles The technological approaches applied for ancient DNA study can also be used for forensic genetic analysis in difficult cases where only microscopic amounts of material are available or the DNA has been severely damaged. Some of these approaches were used in the genetic expertise of the putative remains of the family of the last Russian Emperor Nicholas II Romanov. There is historical evidence that Prince Alexey suffered from severe bleeding that is characteristic of hemophilia. It is now known that hemophilia is caused by insufficient activity of blood clotting factors. Factor VIII deficit caused by mutations in the F8 gene is the cause of the most common hemophilia A (about one in 5000 boys is born with this disease), and Factor IX deficit causes hemophilia B (F9 gene, located on the X- chromosome), which occurs in one in 25000. Your task is to analyze the sequence of the F9 gene and identify the exact cause of the Hemophilia B in the royal family. DOI: 10.32607/20758251-2009-1-3-58-69 Your task is to identify what caused hemophilia B in Alexey. To do so, you need to analyze Alexandra's Olga's, Tatiana's, Maria's, Anastasia's and Alexey's F9 gene: I. Create the primers to amplify the gene of interest. II Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel Subclone the gene into plasmid, extract the plasmid DNA Send the Plasmid DNA to be sequenced (Sanger sequencing), analyze the results (autoradiograms and chromatograms) Identify the mutation(s), speculate on how they might affect the protein function IV. Tsar Nicholas il Friedrich Tsarina Alexandra Anastasia Maria Alexey

Answer 1

The cause of Hemophilia B in Alexey is due to X-linked recessive inheritance as the mother is a carrier and the father is normal. The genes associated with this condition is located on the X chromosome. The carrier X chromosome from the mother is transferred to Alexey and in case of males only one X chromosome is sufficient to cause the disease.