Question

The technological approaches applied for ancient cal approaches applied for ancient DNA study can also be used for forensic genetic analysis in Where only microscopic amounts of material are available or the DNA has been severely damaged. Some of these approaches were used in the genetic expertise of the putative remains on the last Russian Emperor Nicholas II Romanov. is historical evidence that Prince Alexey suffered from severe bleeding that is characteristic of philia. It is now known that hemophilia is caused by insufficient activity of blood clotting factors. Factor Ich caused by mutations in the F8 gene is the cause of the most common hemophilia A (about one in JVU boys is born with this disease), and Factor IX deficit causes hemophilia B (F9 gene, located on the chromosome), which occurs in one in 25000. Your task is to analyze the sequence of the gene and identify the exact cause of the Hemophilia B in the royal family. DOI: 10.32607/20758251-2009-1-3-58-69. Your task is to identify what caused hemophilia B in Alexey. To do so, you need to analyze Alexandra's, Olga's, Tatiana's, Maria's, Anastasia's and Alexey's F9 gene: 1. II. Create the primers to amplify the gene of interest. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. Subclone the gene into plasmid, extract the plasmid DNA. Send the Plasmid DNA to be sequenced (Sanger sequencing), analyze the results (autoradiograms and chromatograms) Identify the mutation(s), speculate on how they might affect the protein function. IV. V. Tsar Nicholas II Friedrich Tsarina Alexandra Tatiana Anastasia Maria Alexey Olga

Answer 1

The exact cause is the X linked gene. This gene is F9. The F9 gene is responsible for synthesis of the protein which are involved in blood clotting. The wild type gene is dominnat over recessive allele. Let dominant allele is XH and the recessive allele is Xh.

Please look in the image for genotypes of the individual:

Tsar Nicholas II XHY XhΥ XHY - XHXh XHXH Friedrich Tsarina Alexandra XHXH XHXH XHXH XHXH XHY Tatiana Maria Anastasia Olga Alexey