Question

Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two different approaches you can take (each using three steps/dilutions).

Could someone please guide me through the steps of how to solve this dilution problem? I'm at a loss as to where to start

Answer 1

EXPLANATION;

A serial dilution is any dilution in which concentration decreases by the same factor in each successive step.

In serial dilutions, You multiply the dilution factors for each step.

The dilution factor or the dilution is the initial volume divided by final volume.

Therefore , Dilution factor (DF) =Vi/Vf

so, to get a dilution of 1:5000, initial volume /final volume =1/5000

Take three test tubes along with a stock solution . Let us consider three test tubes as A B and C . Now transfer 1ml from stock solution to test tube A which already consists of 9ml and continue the process with remaining two tubes so that u can get a final dilution factor as 1:5000