I have a solution of a plasmid at 400ng/uL. I want 20ng of plasmid. Outline the...
Homework Answers
Answer:
- The convergence of plasmid in the given arrangement is 400ng/uL and subsequently a 20 times weakening will give you a grouping of 20ng/uL.
- Take 1ul stock arrangement and include 19 uL of sterile water/1xTE cradle will give 20ng plasmid in 1uL.
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have a solution of a plasmid at 400ng/uL. I want 20ng of plasmid.
Outline the...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To...
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To do a serial dilution of 10% concentration (not volume) for each dilution, how many uL of liquid do you move between each well? ype your answer as a whole number of uL, but do not type the "uL" label. 2) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. What is the final concentration of well 6 if you...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Starting from a 500 ng/uL stock solution, three 1:10 serial dilutions are prepared. What is the...
Starting from a 500 ng/uL stock solution, three 1:10 serial dilutions are prepared. What is the concentration of the last dilution? Write down the answer answer with one digit before and after the decimal place by assuming the units are in ng/uL (for example like. 2.0). Wouldnt it just be 125? Since you just divide by 2 each time?
you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1...
you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1 ug/ul? make your final volume 0.1 ml
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted...
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted to dilute this solution to make a total of 1 ml of a 3 mM solution, what volume of stock and what volume of water would you use? Give your answer in microliters (ul), and show your calculations. Volume of stock3 ul Volume of water · T ul
You have prepared some plasmid DNA in lab and need to know its concentration so you...
You have prepared some plasmid DNA in lab and need to know its concentration so you can load 1 ug on a gel. You dilute 1 uL with 99 uL of water and the resulting sample has an absorbance of 0.075. What volume of the original sample do you need to load onto the gel? (Show math for partial credit and make sure you are answering the question I am actually asking.)
calculate how many microliters of volume are needed to have 1 microgram of each plasmid. HIS...
calculate how many microliters of volume are needed to have 1 microgram of each plasmid. HIS plasmid: 558 ng/ul LEU plasmid: 668 ng/ul 1a plasmid: 910 ng/ul 2a plasmid: 918 ng/ul RNA3-AUUU plasmid: 1086 ng/ul
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted to dilute this solution to make a total of 1 ml of a 3 mM solution, what volume of stock and what volume of water would you use? Give your answer in microliters (ul), and show your calculations. Volume of stock3 ul Volume of water · T ul
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