calculate how many microliters of volume are needed to have 1 microgram of each plasmid. HIS...
calculate how many microliters of volume are needed to have 1 microgram of each plasmid.
HIS plasmid: 558 ng/ul
LEU plasmid: 668 ng/ul
1a plasmid: 910 ng/ul
2a plasmid: 918 ng/ul
RNA3-AUUU plasmid: 1086 ng/ul
Homework Answers
mass of each plasmid = 1 ug = 1000 ng
(a.) HIS plasmid
volume = (mass of plasmid) / (concentration of plasmid)
volume = (1000 ng) / (558 ng/uL)
volume = 1.79 uL
(b.) LEU plasmid
volume = (mass of plasmid) / (concentration of plasmid)
volume = (1000 ng) / (668 ng/uL)
volume = 1.50 uL
(c.) 1a plasmid
volume = (mass of plasmid) / (concentration of plasmid)
volume = (1000 ng) / (910 ng/uL)
volume = 1.10 uL
(d.) 2a plasmid
volume = (mass of plasmid) / (concentration of plasmid)
volume = (1000 ng) / (918 ng/uL)
volume = 1.09 uL
(e.) RNA3-AUUU plasmid
volume = (mass of plasmid) / (concentration of plasmid)
volume = (1000 ng) / (1086 ng/uL)
volume = 0.921 uL
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calculate how many microliters of volume are needed to have 1
microgram of each plasmid.
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