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How are we getting the volumes in this solution for example
for 10Xs buffer how did...
How are we getting the volumes in this solution for example for 10Xs buffer how did we get 1 microliter please explain for each reagent how the microliters were obtained; ddH20, 10X buffer A, 1 micro...
How are we getting the volumes in this solution for example
for 10Xs buffer how did we get 1 microliter please explain for each
reagent how the microliters were obtained; ddH20, 10X buffer A, 1
microgram/microliter DNA stock, and enzyme at 10
U/microliter.
Question: What is the most concentrated stock of buffer A you can make? Now if you want to prepare an enzymatic reaction containing 1X buffer A, 100 ng/ul DNA stock and 1 Unit/ul (U/ul) of enzyme proceed...
You want to digest the phage genomic DNA you just isolated with a restriction enzyme called...
You want to digest the phage genomic DNA you just isolated with a restriction enzyme called HaeIII. You find the enzyme and a 10X stock of HaeIII enzyme buffer in the freezer. How much buffer will you add if the total volume of your reaction is 50 microliters?
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of...
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of DNA digested per hour u to ensure complete digestion), 5X reaction butter, (b-e) to calculate how to prepare a restriction enzym a. Using the A260, what is the DNA's concentration? na miniprep and wish to digest it with EcoRI. You have DNA with an A260 of 2.0, EcoRI at digested per hour under optimal conditions, but 10 units are usually used per reaction "...
Please show all work the above reaction mentioned in question 3 is part of question 2...
Please show all work
the above reaction mentioned in question 3 is part of question
2
3. Suppose the weight of the plasmid is 5000 basepairs (bp) in size. Assuming each base pair has a molecular weight of 600, how many moles of plasmid are present in the above reaction? Picomoles? Femtomoles? (1 pt) 2. Given a 50 ug/mL stock solution of plasmid DNA and a 0.2 U/L enzyme stock, how would you set up a 50 uL reaction containing...
You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing...
You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing your plasmid (concentration = 500 ng/μl) with EcoRI, which is provided in a standard 50% glycerol solution at 10 units/μl. Your enzyme also comes with an appropriate 10X reaction buffer. Taking into account the final allowable glycerol concentration, you want to add the maximum amount of EcoRI, to achieve complete digestion of 1 μg plasmid DNA in a total volume of 20 μl. Fill...
The composition of a 10X restriction enzyme buffer us: 500mM Potassium Acetate; 200 mM Tris-acetate; 100mM...
The composition of a 10X restriction enzyme buffer us: 500mM Potassium Acetate; 200 mM Tris-acetate; 100mM Mg Acetate; 1 mg/ml BSA. a) If you set up a reaction with 25 uL final volume, what volume of 10X buffer would you add to obtain 1X? b) In reference to the above, what is the final concentration of BSA in the 25 uL reaction?
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme...
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme Eco R1. A map of the plasmid is shown here. The entire plasmid is 6000 bp, and there are Eco R1 restriction sites at 1500 bp, 2000 bp, and 4000 bp. You’re going to run the entire volume of the digest on a gel, and you want to cut just enough DNA to have 50 ng in the smallest band on your gel. Starting...
How to solve for question#6? 5 5. Fill in the table below to set up the...
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine...
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
what do you think she should change in her protocol to avoid the problem ? Problem.3:...
what do you think she should change in her protocol to avoid
the problem ?
Problem.3: A student was working on PCR and she performs the additives shown in (A) and get the result in (B): B. Component Volume per reaction (ul) 10X PCR buffer 2.5 MgSO4 dNTPs (10mM) Enzyme Forward Primer (10 pmale) BCH361 (Practicall Biochemistry department Reverse Primer (10 pmele) RNase/DNase-free water 14.8 DNA 4 ul Total volume 25
How are we getting the volumes in this solution for example
for 10Xs buffer how did we get 1 microliter please explain for each
reagent how the microliters were obtained; ddH20, 10X buffer A, 1
microgram/microliter DNA stock, and enzyme at 10
U/microliter.
Question: What is the most concentrated stock of buffer A you can make? Now if you want to prepare an enzymatic reaction containing 1X buffer A, 100 ng/ul DNA stock and 1 Unit/ul (U/ul) of enzyme proceed...
7. You purify DNA from a miniprep and wis units/ml (1 unit = 1 hg of DNA digested per hour u to ensure complete digestion), 5X reaction butter, (b-e) to calculate how to prepare a restriction enzym a. Using the A260, what is the DNA's concentration? na miniprep and wish to digest it with EcoRI. You have DNA with an A260 of 2.0, EcoRI at digested per hour under optimal conditions, but 10 units are usually used per reaction "...
Please show all work
the above reaction mentioned in question 3 is part of question
2
3. Suppose the weight of the plasmid is 5000 basepairs (bp) in size. Assuming each base pair has a molecular weight of 600, how many moles of plasmid are present in the above reaction? Picomoles? Femtomoles? (1 pt) 2. Given a 50 ug/mL stock solution of plasmid DNA and a 0.2 U/L enzyme stock, how would you set up a 50 uL reaction containing...
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
what do you think she should change in her protocol to avoid
the problem ?
Problem.3: A student was working on PCR and she performs the additives shown in (A) and get the result in (B): B. Component Volume per reaction (ul) 10X PCR buffer 2.5 MgSO4 dNTPs (10mM) Enzyme Forward Primer (10 pmale) BCH361 (Practicall Biochemistry department Reverse Primer (10 pmele) RNase/DNase-free water 14.8 DNA 4 ul Total volume 25
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