what do you think she should change in her protocol to avoid the problem ? Problem.3:...

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what do you think she should change in her protocol to avoid the problem ?
Problem.3: A student was working on PCR and she performs the additives shown in (A) and get the result in (B): B. Component V

Problem.3: A student was working on PCR and she performs the additives shown in (A) and get the result in (B): B. Component V

science biology

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The gel shows non specific pcr product , these may occur due to DAN contamination ( during lab work,or DNA not free from proteins and RNase). Water not free contamination.To aviody these ensure sterlie condition in lab when you are working, use gloves etc.ensure DNA free contamination.Correct amont of master mix.If don't get result in repeated attempts please change the master mix (previously used).

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