Question

The composition of a 10X restriction enzyme buffer us: 500mM Potassium Acetate; 200 mM Tris-acetate; 100mM Mg Acetate; 1 mg/ml BSA.

a) If you set up a reaction with 25 uL final volume, what volume of 10X buffer would you add to obtain 1X?

b) In reference to the above, what is the final concentration of BSA in the 25 uL reaction?

Answer 1

Answer

a) for dilution of 10X buffer into 1 X buffer means 10-time dilution

so 1 ul of 10X buffer will be added into 9 ul of water to get 1X 10ul buffer

so if the volume is 25ul then 25/10 =2.5 ul

therefore, 2.5 ul of 10 X buffer needed for making 25 ul of 1X reaction buffer.

b) the stock concentration of BSA is 1mg /ml, means 1ug/ul

in the 2.5 ul of 10X buffer will have 2.5ug of BSA in the 25 ul of 1X reaction buffer.