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Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; t...
7. The DNA (or RNA) sample loading buffer contains Tris, acetate and EDTA and has a...
7. The DNA (or RNA) sample loading buffer contains Tris, acetate and EDTA and has a pH of 8.0. In addition, the loading buffer also contains 5% glycerol and bromophenol blue. Tris and acetate are buffer to control the solution pH at 8.0, EDTA is a DNase inhibitor which prevents DNA from digesting by DNase. So, what are the purposes of having 5% glycerol and bromophenol blue in the sample loading buffer?
I need help doing problem number 5 listed below. If someone could show the work required...
I need help doing problem number 5 listed below. If someone could show the work required for this problem it would be a great help! Below is a list of solutions you will need for the mini-prep. Make 5mL of each solution as a team and store each in a 15mL Falcon tube. Store solution 1 and 70% ethanol at 4 degrees Celsius. Solution 3 and Elution Buffer can be stored at room temperature. Show your calculations in the space...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
6. How many mL of 0.2 M sodium acetate should be added to 200 mL of...
6. How many mL of 0.2 M sodium acetate should be added to 200 mL of 0.2 M acetic acid to make a buffer of pH 5.5? (refer to Table 2-2 on page 60 for pKa values). What is the molarity of the resulting buffer with respect to acetate (acetate + acetic acid)? 7. How many mL of 0.2 M HCl should be added to 50 mL of 0.2 M Tris base (Trishydroxymethyl aminomethane) to make a buffer of pH...
3. A common buffer used in working with DNA solutions is tris. tris a) Draw the...
3. A common buffer used in working with DNA solutions is tris. tris a) Draw the structure of the protonated form of tris. HO Но, b) When 30.00 mL of a 62.20 mM tris solution is titrated with 0.100 M HCl, the pH equals 7.40 after 3.96 mL of HCl are added. What is the PK of tris? c) (Optional, not counted) How would you prepare 500.0 mL of a 0.0100 M PH 7.4 tris buffer solution assuming you had...
You need to prepare an acetate buffer of pH 6.29 from a 0.704 M acetic acid...
You need to prepare an acetate buffer of pH 6.29 from a 0.704 M
acetic acid solution and a 2.48 M KOH solution. If you have 575 mL
of the acetic acid solution, how many milliliters of the KOH
solution do you need to add to make a buffer of pH 6.29? The pKa of
acetic acid is 4.76.
You need to prepare an acetate buffer of pH 6.29 from a 0.704 M acetic acid solution and a 2.48 M...
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps...
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body. A) You need to prepare a stock solution at pH 7.00 with KH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2 ] + [H2PO4 − ] = 0.3...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
Question 6 Using stock solutions to make up a solution: from final concentration to volume You...
Question 6 Using stock solutions to make up a solution: from final concentration to volume You need to digest a sample of DNA with a restriction enzyme. You will do this reaction later in the semester. The buffer for this reaction has a final concentration of 40 mM Tris, pH 7.5, 10 mM MgCl2 and 50 mM KCl. You have the following stock solutions: 0.5 M Tris, pH 7.5, 1 M MgCl2 and 2 M KCl. How would you make...
You need to prepare an acetate buffer of pH 6.25 from a 0.896 M acetic acid...
You need to prepare an acetate buffer of pH 6.25 from a 0.896 M acetic acid solution and a 2.46 M KOH solution. If you have 680 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 6.25? The pK, of acetic acid is 4.76. ml
7. The DNA (or RNA) sample loading buffer contains Tris, acetate and EDTA and has a pH of 8.0. In addition, the loading buffer also contains 5% glycerol and bromophenol blue. Tris and acetate are buffer to control the solution pH at 8.0, EDTA is a DNase inhibitor which prevents DNA from digesting by DNase. So, what are the purposes of having 5% glycerol and bromophenol blue in the sample loading buffer?
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
6. How many mL of 0.2 M sodium acetate should be added to 200 mL of 0.2 M acetic acid to make a buffer of pH 5.5? (refer to Table 2-2 on page 60 for pKa values). What is the molarity of the resulting buffer with respect to acetate (acetate + acetic acid)? 7. How many mL of 0.2 M HCl should be added to 50 mL of 0.2 M Tris base (Trishydroxymethyl aminomethane) to make a buffer of pH...
3. A common buffer used in working with DNA solutions is tris. tris a) Draw the structure of the protonated form of tris. HO Но, b) When 30.00 mL of a 62.20 mM tris solution is titrated with 0.100 M HCl, the pH equals 7.40 after 3.96 mL of HCl are added. What is the PK of tris? c) (Optional, not counted) How would you prepare 500.0 mL of a 0.0100 M PH 7.4 tris buffer solution assuming you had...
You need to prepare an acetate buffer of pH 6.29 from a 0.704 M
acetic acid solution and a 2.48 M KOH solution. If you have 575 mL
of the acetic acid solution, how many milliliters of the KOH
solution do you need to add to make a buffer of pH 6.29? The pKa of
acetic acid is 4.76.
You need to prepare an acetate buffer of pH 6.29 from a 0.704 M acetic acid solution and a 2.48 M...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
You need to prepare an acetate buffer of pH 6.25 from a 0.896 M acetic acid solution and a 2.46 M KOH solution. If you have 680 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 6.25? The pK, of acetic acid is 4.76. ml
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