3. A common buffer used in working with DNA solutions is tris. tris a) Draw the...
5. Tris i.e. [tris(hydroxymethyl)aminomethane) is commonly used as a buffer in bioanalytical studies. The pk of Tris is 8.1 at 20°C. (a) Calculate the pH of the Tris buffer after mixing 12.0 mL of 0.15 M of a HCl solution with 20.0 mL of a 0.20 M solution of Tris. (5 points) (b) An amount of 0.00017 mol of H+ was consumed during an enzymatic reaction. What is the pH of the buffer at the end of the reaction? (5...
21.) Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by a biological chemist using 10.0M NaOH to a pH of 7.79 using 31.52 g of (HOCH2)3CNH3Cl, or TrisH+, (MW=157.597g/mol, pKa 8.075). >>>> Need help with second part of question pls>>>>...Now you dilute the solution from problem 21 to 1.0 L and take half of the buffer (500.0 mL) and add 0.0100 moles solid HCl. What is the new pH?
Tris buffers are commonly used in biochemistry labs because they buffer within the physiological range of 7.1 to 9.1 due to a pKa of 8.1. To demonstrate the buffering capacity of Tris buffer, your biochemistry lab teaching assistant has given you one liter of a 0.1 M Tris buffer at pH 7.4. Add 2 mL of 1M HCl to this buffer and calculate what the new pH will be.
Part A Tris[tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (Ka = 5.01 × 10−9 and pKa = 8.3) (a) Calculate the pH of the Tris buffer after mixing 13.5 mL of 0.15 M HCl solution with 25.0 mL of 0.10 M Tris. (b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00017 mol of H+ was produced. What is the pH of the buffer at the end of the reaction? (c)...
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body. A) You need to prepare a stock solution at pH 7.00 with KH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2 ] + [H2PO4 − ] = 0.3...
Show calculations for the solutions.
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
3. MOPS-3(N-morpholino)propanesulfonic acid (structure below) is a common buffer used in biochemistry and biology. Consider a 0.100 M MOPS/Na-(MOPS) buffer solution (200.0 mL). pk. (MOPS) = 7.20 a. Calculate the pH of the buffer. b. Calculate the pH when 1.0 mL of 1.0 M NaOH is added to the buffer. C. Calculate the pH when 5.0 mL of 1.0 M NaOH is added to the buffer. d. Calculate the pH when 10.0 mL of 1.0 M NaOH is added to...
3) Kb for NH3 1.8 x 10-5 a) Calculate the pH of a buffer solution that is 0.100 M NH3 and 0.120 M NHANO3. b) What is the pH after 60.0 mL of 0.010 M HCl is added to 90.0 mL of the buffer solution in 2(a). Assume the change in volume is additive. c) What is the pH after 60.0 mL of 0.010 M Ca(OH)2 is added to 90.0 mL of the buffer solution in 2(a). Assume the change...
Calculate the pH expected for the 0.0100 M HCl solution
used in part A.
I'm so lost please help
Intermediate value Final value 1a. Calculate the pH expected for the 0.0100 M HCl solution used in part A. 1b. Calculate the percent error between the expected pH and your measured pH of the 0.0100 M HCI. our To ☺ QUESTION 2 Intermediate value Final value 2a. Calculate the pH of 0.100 M CH3COOH (approximation method). Kg = 1.8 x 10-5...
MOPS – 3(N-morpholino)propanesulfonic acid (structure below) is a common buffer used in biochemistry and biology. Consider a 0.100 M MOPS/Na+•(MOPS-) buffer solution (200.0 mL). pKa (MOPS) = 7.20 (0.1 M MOPS AND 0.1 M Na+ MOPS-) a. Calculate the pH of the buffer. b. Calculate the pH when 1.0 mL of 1.0 M NaOH is added to the buffer. c. Calculate the pH when 5.0 mL of 1.0 M NaOH is added to the buffer.