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Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent ...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1...
Pre
lab quiz question
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the sample for a tube using 0.6 mL of the 2.0 mg/ml standard BSA Your Answer: Answer units
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples:...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Pre
lab quiz question
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the sample for a tube using 0.6 mL of the 2.0 mg/ml standard BSA Your Answer: Answer units
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
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