4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...

Question

Question

4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and

Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your work for the calculations to receive

Answer 1

Answer #1

Concentration versus absorbance data is plotted:

With the equation of the line:

Y = 0.162 * X + 0.0173

The concentration of the unknown is calculated:

0.083 = 0.162 * X + 0.0173

X is cleared = 0.406 mg / mL

If you liked the answer, please rate it in a positive way, you would help me a lot, thank you.

Answer 2

Similar Homework Help Questions

ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...

ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
4) An unknown concentration of NiSO4 solution is to be determined by colorimetric analysis using a...

4) An unknown concentration of NiSO4 solution is to be determined by colorimetric analysis using a spectrophotometer. The standard solutions are prepared as the following: 2.00 mL (the same procedures for 3.0 mL, 4.00 mL, 6.00 mL, 7.00 mL) of the stock solution of 1.00 M NİSO4 is transferred into a 25-mL volumen flask and diluted by adding distilled water to the mark line. The spectrophotometer is calibrated at the wavelength of 635 nm. The absorbance (A) readings and the...
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a sol...

If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?

If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg...

If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
Complete the purification table below for that group using the information supplied in Tables 1 &...

Complete the purification table below for that group using the information supplied in Tables 1 & 2. You may refer to the description in your lab manual on how to calculate these parameters and account for any dilutions. Sample Activity Total (ABS/min/mL) activity (ABS/min OR U) Protein Concentration (mg/mL) Total Protein (mg) Specific activity (ABS/min/mg OR U/mg) Fold Purification RP PPE [28 marks] Table 1: Triplicate absorbance data of Group A for the catechol assay of banana PPO after 2...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...

Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...

A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...

A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
The osmotic pressure of a solution containing 22.7 mg of an unknown protein in 50.0 mL...

The osmotic pressure of a solution containing 22.7 mg of an unknown protein in 50.0 mL of solution is 2.88 mm Hg at 25.0°C. Determine the molar mass of the protein. Select one: O a. 3.85 x 10 g/mol O b.3.85 g/mol O c. 2.46 x 10 g/mol O d. 1.47 x 10 g/mol e. 2.93 x 109 g/mol
lab question 1. What is the basis of the different purification methods? 2. What are some...

lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...

Please help! Ligand X forms a complex with both cobalt and copper, cach of which has...

Please help! Ligand X forms a complex with both cobalt and copper, cach of which has a maximum absorbance at 510 nm and 645 nm, respectively. A 0.217 g sample containing cobalt and copper was dissolved and diluted to a volume of 100.0 mL. A solution containing ligand X was added to a 50.0 mL aliquot of the sample solution and diluted to a final volume of 100.0 mL. The measured absorbance of the unknown solution was 0.493 at 510...