If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg...
- If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
Homework Answers
Measuring absorbance to calculate the protein concentration is a
simple and fast technique. Often, proteins absorb at 280 nm in UV
spectroscopy because of aromatic rings. So the calculation is
similar to Beer-Lambert's law.
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If the molecular weight of Protein G:B1 is 8 kDa, what is the
concentration (in mg...
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a sol...
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
29. Complete the problem on the slide titled 'Sample Problem". If the molecular weight of Protein G:B1 is 8 kDa, w...
29. Complete the problem on the slide titled 'Sample Problem". If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg/mL) of a solution of this protein which has an absorbance (A) =0.2 at 2 = 280 nm? How many grams of protein are present in 1 mL of this solution? Protan G:B1 3 tyrosine & One tryptophan
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
Please answer both 1. Often when handling proteins, we use concentration in terms of mg/ml. A...
Please answer both 1. Often when handling proteins, we use concentration in terms of mg/ml. A high concentration is greater than 1 mg/ml and low concentrations are below 0.1 mg/ml (roughly). If you obtained a sample of MDH that was 10 micromoles/L, what is the concentration in mg/ml? Assume that the molecular weight for MDH is 32 kDa. 2. Using Beer's Law, determine the concentration of your sample if the UV-vis reading at 280 nm was 1.2 absorbance units and...
The molecular mass of a protein is 50000 Da. (A) What is the mg/mL concentration of...
The molecular mass of a protein is 50000 Da. (A) What is the mg/mL concentration of a 40-µM solution of the protein? (B) What is the percentage concentration of this solution?
A given protein solution has a concentration of 5 mg/mL, and the molecular mass of the...
A given protein solution has a concentration of 5 mg/mL, and the molecular mass of the protein is 25000 Da. (A) What is the molarity [M] of the solution? (B) What volume is needed to make 150 µL at 100 µM?
You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular...
You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular weight of 22,680 g/mol is 75 µM. What is the concentration in mg/ml?
FliC is a protein involved in bacterial flagella motion. It has a molecular weight of 43,545...
FliC is a protein involved in bacterial flagella motion. It has a molecular weight of 43,545 Da and an extinction coefficient of 48,299. You have been able to purify this protein, and find that your 3 mL sample has an OD280 of 0.592. Length of the cuvette is 1. What is the concentration of FliC? A) 12.3 uM How many nanomoles of FliC do you have? A) 36.8 nM Im not sure how they got 12.3 uM and then to...
If the molar absorptivity at a specific wavelength is known for a protein, it is pretty...
If the molar absorptivity at a specific wavelength is known for a protein, it is pretty easy to determine its concentration simply by measuring its absorbance and using Beers law. Based on the following information, spectrophotometrically determine the concentration (in mg/mL) of a solution of the protein lysozyme. -the molar absorptivity at 280 nm (converted for units of mg/mL) is & = 0.243 mg.cm -the absorbance of a solution of lysozyme is 0.923 when the path length is 1 cm.
This is for a biochemistry class. Thanks 7. How is phenyl Isothiocyanate useful in determining protein...
This is for a biochemistry class. Thanks
7. How is phenyl Isothiocyanate useful in determining protein structure? 8. The absorption coefficient of bovine immunoglobulin at 280 nm is 210,000 M cm What is the absorbance of a 0.1 mg ml solution across a 1-cm path? What percentage of the incident light is transmitted by this solution? (Hint: check question 3.4 in the textbook) 9. The relative electrophoretic mobilities of a 30-kDa protein and a 92 kDa protein used as standards...
29. Complete the problem on the slide titled 'Sample Problem". If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg/mL) of a solution of this protein which has an absorbance (A) =0.2 at 2 = 280 nm? How many grams of protein are present in 1 mL of this solution? Protan G:B1 3 tyrosine & One tryptophan
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
If the molar absorptivity at a specific wavelength is known for a protein, it is pretty easy to determine its concentration simply by measuring its absorbance and using Beers law. Based on the following information, spectrophotometrically determine the concentration (in mg/mL) of a solution of the protein lysozyme. -the molar absorptivity at 280 nm (converted for units of mg/mL) is & = 0.243 mg.cm -the absorbance of a solution of lysozyme is 0.923 when the path length is 1 cm.
This is for a biochemistry class. Thanks
7. How is phenyl Isothiocyanate useful in determining protein structure? 8. The absorption coefficient of bovine immunoglobulin at 280 nm is 210,000 M cm What is the absorbance of a 0.1 mg ml solution across a 1-cm path? What percentage of the incident light is transmitted by this solution? (Hint: check question 3.4 in the textbook) 9. The relative electrophoretic mobilities of a 30-kDa protein and a 92 kDa protein used as standards...
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