You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular weight of 22,680 g/mol is 75 µM. What is the concentration in mg/ml?
75 micro molar solution means that 75 micromoles of protein are present in 1 L solution.
We can also say that 22680×75×10^-6 g protien is present in 1L solution.
1.7 g protien present in 1L solution.
1700 mg protien present in 1000 ml solution.
So concentration in mg/ml=1.7
You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular...
The molecular mass of a protein is 50000 Da. (A) What is the mg/mL concentration of a 40-µM solution of the protein? (B) What is the percentage concentration of this solution?
A given protein solution has a concentration of 5 mg/mL, and the molecular mass of the protein is 25000 Da. (A) What is the molarity [M] of the solution? (B) What volume is needed to make 150 µL at 100 µM?
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
If you dissolve 412 mg sucrose (formula weight: 342.29 g/mol) in 60 mL of water: a. What is the concentration of sucrose in mM in µM? b. If you take 5 mL of this solution and dilute it to 250 mL, what is the concentration of the glucose in the resulting solution in µM? c. How many nano moles of sucrose are present in 10 mL of the diluted solution?
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
You want to measure the molecular weight of a protein molecule so you measure the osmotic pressure of a protein solution you make. If 0,10 g of protein is dissolved in 5.0 ml. of solution, n = 0.05 atm at 25°C. What is the molecular weight of the protein? 820 g/mol Anamn
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
If you dissolve 412 mg sucrose (formula weight: 342.29 g/mol) in 60 mL of water: a. What is the concentration of sucrose in mM in µM? b. If you take 5 mL of this solution and dilute it to 250 mL, what is the concentration of the glucose in the resulting solution in µM? c. How many nano moles of sucrose are present in 10 mL of the diluted solution?
Calculate the concentration in mol/L, M, of an aqueous sugаr solution with a concentration of 22.5% (w/w) and density of 1.070 g/mL at 25°C. The molecular weight of sugar is 342.30 g/mol. Report your answer to three significant figures. М.
3. Concentrations in solids and solution. You send a 10-g sample of rice to the lab for the determination of the total arsenic content. The analyst dissolves 1.0 g of the rice in 5.0 mL of concentrated acid and then dilutes the solution to a final volume of 25.0 mL. When analyzed by ICP-MS, the solution concentration is found to be 35 µg L-1. What is the concentration of arsenic in the rice (in µg kg-1)? ___________ ppb Hint: Follow...