A given protein solution has a concentration of 5 mg/mL, and the molecular mass of the...
The molecular mass of a protein is 50000 Da. (A) What is the mg/mL concentration of a 40-µM solution of the protein? (B) What is the percentage concentration of this solution?
You calculate via spectrophotometry that the concentration of a solution of a protein with a molecular weight of 22,680 g/mol is 75 µM. What is the concentration in mg/ml?
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
If the molecular weight of Protein G:B1 is 8 kDa, what is the concentration (in mg / mL) of a solution of this protein which has an absorbance (A) =0.2 at l = 280 nm? How many grams of protein are present in 1 mL of this solution?
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
A technologist is ask to prepare 100 mL of a 5% (v/v) concentration of sodium hypochloride. How much solute should they add to the concentrated sodium hypochloride? A CLS needs to make a 1 to 10 dilution with a total volume of 100.0 mu L. How many mu L of patient serum must be used to make this dilution? Consider if you wanted to make 1.00 L of a 0.200 M solution of Na3PO4 (163.94 g). How many grams of....
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
1. The LD50 of cocaine is 95.1 mg/kg. Calculate the concentration of a solution, in molarity if they drank 1 liter of it. 100 g / 303.346 g/mol = 0.329 mol of cocaine. 0.329 mol/ 1 L= 0.329 M for molarity. Is this correct? 2. Find one other concentration unit [mass/mass (g/g) or mass/volume (g/ml) percentage, ppm or ppb] that would be toxic to a 85 kg person. Assume the density of a solution of your substance is the same...