Homework Answers
Density of protein (standard) = 1.350 g/ml
Density of biuret reagent = 1.4671g/ml
Thus, mass of protein used = 2.025 gms
Mass of biuret reagent used = 2.2005 gms
Mass fraction of protein in made up sample= 0.48
Concentration of protein in std bsa = 0.48*2 = 0.96 mg/ml
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Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent ...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
Complete the purification table below for that group using the information supplied in Tables 1 &...
Complete the purification table below for that group using the information supplied in Tables 1 & 2. You may refer to the description in your lab manual on how to calculate these parameters and account for any dilutions. Sample Activity Total (ABS/min/mL) activity (ABS/min OR U) Protein Concentration (mg/mL) Total Protein (mg) Specific activity (ABS/min/mg OR U/mg) Fold Purification RP PPE [28 marks] Table 1: Triplicate absorbance data of Group A for the catechol assay of banana PPO after 2...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5...
please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5 mL ep tube, do you think you can make 2.0 mg BSA / 1 mL Water concentration sample in a 1.5 mL ep tube? If so, please explain to me. If not, please explain to me why.
I need help ASAP pleaseeee it is due in 30mins Question 5 (0.5 points) If you...
I need help ASAP pleaseeee it is due in 30mins
Question 5 (0.5 points) If you added a enzyme to a protein sample in which the polymers were degraded into individual amino acids, what would you expect after adding Biuret reagent? OA) A deep violet purple color would be present B) Enzymes cannot be used to break down proteins C) There would be no reaction and thus no color change D) The color of the solution would reflect the concentration...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
Complete the purification table below for that group using the information supplied in Tables 1 & 2. You may refer to the description in your lab manual on how to calculate these parameters and account for any dilutions. Sample Activity Total (ABS/min/mL) activity (ABS/min OR U) Protein Concentration (mg/mL) Total Protein (mg) Specific activity (ABS/min/mg OR U/mg) Fold Purification RP PPE [28 marks] Table 1: Triplicate absorbance data of Group A for the catechol assay of banana PPO after 2...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
I need help ASAP pleaseeee it is due in 30mins
Question 5 (0.5 points) If you added a enzyme to a protein sample in which the polymers were degraded into individual amino acids, what would you expect after adding Biuret reagent? OA) A deep violet purple color would be present B) Enzymes cannot be used to break down proteins C) There would be no reaction and thus no color change D) The color of the solution would reflect the concentration...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
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