please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5...
please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5 mL ep tube, do you think you can make 2.0 mg BSA / 1 mL Water concentration sample in a 1.5 mL ep tube? If so, please explain to me. If not, please explain to me why.
Homework Answers
If ep tube is of 1.5 ml and yes we can achieve 2 mg/mL concentration.
If we dissolve 3 mg in 1.5 ml then we can achieve 2 mg/mL concentration in 1.5ml ep tube.
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please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Please show work: 1) Complete the calculations and fill in the missing volumes needed below to...
Please show work:
1) Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 1.0 mg/ml BSA (bovine serum albumin) into 1.5 ml microcentrifuge tubes using CIVI = C2V2, note V2 is not 1.5ml. A table is provided below to record your efforts. New solutions Tube Concentration Volumes stock protein Dl water Final volume 1.00 mg/ml 250.0 ul 0.0 ul 250.0 ul 250.0 ul 0.80 mg/ml 0.60...
concentration of the stock is 39.2mg 3. (6 pts) Figure out your plan for making each...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
You find yourself setting up another Western blot, however. today you are preparing the samples t...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
Please type your answer so I can read it, and I just need help for question...
Please type your answer so I can read it, and I just
need help for question one ( Hypothesis ) don't worry about the
rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples:...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Please show work:
1) Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 1.0 mg/ml BSA (bovine serum albumin) into 1.5 ml microcentrifuge tubes using CIVI = C2V2, note V2 is not 1.5ml. A table is provided below to record your efforts. New solutions Tube Concentration Volumes stock protein Dl water Final volume 1.00 mg/ml 250.0 ul 0.0 ul 250.0 ul 250.0 ul 0.80 mg/ml 0.60...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
Please type your answer so I can read it, and I just
need help for question one ( Hypothesis ) don't worry about the
rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
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