Question

Please type your answer so I can read it, and I just need help for question one ( Hypothesis ) don't worry about the rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as secondary standards. Note: 15 g/L tube has been completed and calculation steps are provided below. C1V1 = C2V2 (Where C1 is the initial concentration, V1 is the initial volume, C2 is the final concentration and V2 is the final volume). In this scenario, we know C1 is 60 g/L, C2 is 15 g/L (for making 15 g/L secondary standard) and V2 is 1 mL (1 mL=1000 uL). The unknown factor is V1. 1ml - V1 = volume of water needs to make up 1 mL secondary standard solution (i.e. to make up the final volume as 1 mL). Now apply this formula to the 15 g/L secondary standard tube. C1V1=C2V2 60 g/L x V1 = 15 g/L x 1000 uL V1 = (15 g/L x 1000 uL)/60 g/L V1 = 250 UL. 1000 uL - 250 uL = 750 UL deionised water. You must calculate for 30 g/L and 45 g/L secondary standards and complete Table 1 below. #Table 1: Secondary standards to be used in the Albumin assay. Microfuge Tube 60 g/L Albumin Standard (UL) Deionised water (UL) 15 g/L 250 750 30 g/L 45 g/L

Answer 1

Aim: To study the absorbance of Albumin at different concentrations.

Hypothesis:

In this experiment we are going to observe the absorbance pattern of albumin at different concentrations. For this we have a standard solution of albumin with concentration 60 g/L, which will be our primary standard and we also prepared other 3 standard solutions with concentration 15,30,45 g/L respectively which will be our secondary standard.

These samples were mixed with 2ml of BGC reagent and incubated at room temperature for 5 minutes. They were placed into spectrometer afterwards and with an optimal wavelength of 630 nm, the absorbance pattern were measured.