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ignore the std uncertainty. I need help calculating the mass of Cu g from the concentraion...
I need help filling up this table. Only for the mass protein
calculation. Please show the calculations for at least 2 examples.
Thank you!
Lab #2: Standard Protein Assay Sample or standard (add more rows as Absorbance at Mass protein595 nm Volume , yul appropriate) 0 mg/ml (PBS) 2011 0.125 mg/ml 0.250 mg/ml 0.500 mg/ml 0.750 mg/ml 20 11 20 Lul 2011 20 ul 1.00 mg/ml 1.500 mg/ml 2011 20 Lul 2.000 mg/ml Unknown, 1/10 20 ul 20 ul 20...
need help on these
Suppose you obtained the following data from two standard solutions of known concentrations using a UV-Vis spectroscope: Analysis # Conc. (mg/L) 8.00 20.00 Absorbance (at 255 nm) 0.160 0.390 What is the concentration (in mg/L) of an unknown solution with an absorbance of 0.200 at 255 nm 10 points)? The unknown solution contains the same analyte as the standard solution does (Assume a path length of 1cm). 10. You prepared a stock solution by dissolving a...
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...