Question

I need help filling up this table. Only for the mass protein calculation. Please show the calculations for at least 2 examples. Thank you!

Lab #2: Standard Protein Assay Sample or standard (add more rows as Absorbance at Mass protein595 nm Volume , yul appropriate) 0 mg/ml (PBS) 2011 0.125 mg/ml 0.250 mg/ml 0.500 mg/ml 0.750 mg/ml 20 11 20 Lul 2011 20 ul 1.00 mg/ml 1.500 mg/ml 2011 20 Lul 2.000 mg/ml Unknown, 1/10 20 ul 20 ul 20 ul Unknown, 1/100 20 ul Unknown, 1/1000

Answer 1

First you must calculate the added milliliters, converting the microliters:

mL = uL / ​​1000 = 20 uL / ​​1000 = 0.02 mL

Next, the added milligrams of protein are calculated, for that we take the examples of 0.125 and 0.250 mg / mL:

For 0.125 mg / mL:

Protein mass = 0.125 mg / mL * 0.02 mL = 0.0025 mg

For 0.250 mg / mL:

Protein mass = 0.250 mg / mL * 0.02 mL = 0.0050 mg

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