Homework Answers
Add Answer to:
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each...
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml...
Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml of appropriately diluted enzyme to the cuvette which already contains Tris buffer (1.5 mL) and BTEE (1.4 mL). What volume (in uL) of enzyme does he need for a final reaction concentration of 20 ug/mL?
1. Use metric conversion factors to solve each of the following problems a. A Bee Hummingbird...
1. Use metric conversion factors to solve each of the following problems a. A Bee Hummingbird has a mass of 0.0018 kg. What is the mass of the hummingbird in micrograms (ug)? b. An assay protocol requires you to add 12.5 mg of lysozyme to 1.0 L of buffer. You have a lysozyme stock vial labeled 25 mg/mL. How many microliters (μL) of the stock will you need to add to your solution. Express 0.5 mg/mL in μg/uL Express 0.4...
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock conce...
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
1. Max needs to estimate the protein concentration of a cell lysate. He first read the...
1. Max needs to estimate the protein concentration of a cell lysate. He first read the absorbance of 100 uL aliquots of his sample at 280 nm and calculated an average absorbance of 0.623. Next, using this information he plans to dilute his sample for a Lowry assay. a) Based upon the A280 readings, what was the estimated concentration of Max’s cell lysate? b) Max wants to make 1:2, 1:4, 1:10, and 1:20 dilutions of his cell lysate. Describe what...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
You have a stock solution of FastGreen at a concentration of 10 ug/ml. What volumes of...
You have a stock solution of FastGreen at a concentration of 10 ug/ml. What volumes of solution and water do you need to mix to obtain 400 ul of a 2 ng/ul solution? (Show ALL calculations)
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the most...
Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the most accurate procedure so you end up with at least 500ul each of 5ug/ul, 0.5 ug/ul, and 0.05 ug/ul working solutions of BSA.
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
1. Use metric conversion factors to solve each of the following problems a. A Bee Hummingbird has a mass of 0.0018 kg. What is the mass of the hummingbird in micrograms (ug)? b. An assay protocol requires you to add 12.5 mg of lysozyme to 1.0 L of buffer. You have a lysozyme stock vial labeled 25 mg/mL. How many microliters (μL) of the stock will you need to add to your solution. Express 0.5 mg/mL in μg/uL Express 0.4...
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
You have a stock solution of FastGreen at a concentration of 10 ug/ml. What volumes of solution and water do you need to mix to obtain 400 ul of a 2 ng/ul solution? (Show ALL calculations)
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Most questions answered within 3 hours.
-
Consider the reaction, C3 H8 + O2 --> CO2 + H2O. How many
moles of O2...
asked 1 hour ago -
You and your opponent both roll a fair die. If you both roll the
same number,...
asked 1 hour ago -
In a study of the accuracy of fast food drive-through orders,
Restaurant A had 257 accurate...
asked 1 hour ago -
Identify and describe in detail the four categories of
institutions that could be included in a...
asked 1 hour ago -
In python
class Customer:
def __init__(self, customer_id, last_name, first_name, phone_number, address):
self._customer_id = int(customer_id)
self._last_name =...
asked 1 hour ago -
What is an example of a limitation in implementing a new
ERP system and how it...
asked 1 hour ago -
In a section of 9.7cm of an artery with a radius of 2.6mm there
is a...
asked 1 hour ago -
the two carboxylic acid groups of aspartic acid have different
acidities with pKa values of 2.1...
asked 1 hour ago -
Would CuCO3 aqueous salt combined with calcium chloride
form a solid precipitate? If so, what would...
asked 1 hour ago -
How do ECM Solutions assist in embedding a culture of continuous
improvement in an organization? (Project...
asked 2 hours ago -
Directions
These directions introduce the idea of Essential Questions.
Since this may be a new concept...
asked 2 hours ago -
1.b. Fiscal policy is said to suffer from ‘crowding out’.
Explain what this means and why...
asked 2 hours ago