Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml...
Damon has a stock chymotrypsin concentration of 1 mg/mL. The
protocol states to add 0.1 ml of appropriately diluted enzyme to
the cuvette which already contains Tris buffer (1.5 mL) and BTEE
(1.4 mL).
What volume (in uL) of enzyme does he need for a final reaction
concentration of 20 ug/mL?
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Damon has a stock chymotrypsin concentration of 1 mg/mL. The
protocol states to add 0.1 ml...
Damon has a stock BTEE concentration of 0.00107 M. The protocol states to add 1.4 ml...
Damon has a stock BTEE concentration of 0.00107 M. The protocol states to add 1.4 ml of BTEE to the cuvette, which already contains Tris buffer (1.5 mL) and to which the enzyme will also be added (0.1 mL). What is the concentration of substrate used in the generic protocol? Give your answer in micromolar (uM) and rounded up to the nearest hundred.
concentration of the stock is 39.2mg 3. (6 pts) Figure out your plan for making each...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
The composition of a 10X restriction enzyme buffer us: 500mM Potassium Acetate; 200 mM Tris-acetate; 100mM...
The composition of a 10X restriction enzyme buffer us: 500mM Potassium Acetate; 200 mM Tris-acetate; 100mM Mg Acetate; 1 mg/ml BSA. a) If you set up a reaction with 25 uL final volume, what volume of 10X buffer would you add to obtain 1X? b) In reference to the above, what is the final concentration of BSA in the 25 uL reaction?
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Question 5 Using stock solutions in a protocol: from volume to final concentration A DNA ligation...
Question 5 Using stock solutions in a protocol: from volume to final concentration A DNA ligation reaction is carried out in a 25 μL reaction mix. This is a reaction carried out in cloning. You are going to do a number of these reactions so you decide to make up a larger volume of the reaction mixture, called a master mix. This saves on multiple repetitive pipetting and reduces errors. You have been given a protocol to make up 1...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Question 5 Using stock solutions in a protocol: from volume to final concentration A DNA ligation reaction is carried out in a 25 μL reaction mix. This is a reaction carried out in cloning. You are going to do a number of these reactions so you decide to make up a larger volume of the reaction mixture, called a master mix. This saves on multiple repetitive pipetting and reduces errors. You have been given a protocol to make up 1...
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