Damon has a stock BTEE concentration of 0.00107 M. The protocol states to add 1.4 ml...
Damon has a stock BTEE concentration of 0.00107 M. The protocol states to add 1.4 ml of BTEE to the cuvette, which already contains Tris buffer (1.5 mL) and to which the enzyme will also be added (0.1 mL). What is the concentration of substrate used in the generic protocol? Give your answer in micromolar (uM) and rounded up to the nearest hundred.
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Damon has a stock BTEE concentration of 0.00107 M. The protocol
states to add 1.4 ml...
Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml...
Damon has a stock chymotrypsin concentration of 1 mg/mL. The protocol states to add 0.1 ml of appropriately diluted enzyme to the cuvette which already contains Tris buffer (1.5 mL) and BTEE (1.4 mL). What volume (in uL) of enzyme does he need for a final reaction concentration of 20 ug/mL?
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
The Ka of lactic acid, HC3H5O3, is 1.4 ✕ 10−4. (a) Suppose buffer #1 is prepared using 40.0 mL 0.1 M HC3H5O3 and 60.0 mL...
The Ka of lactic acid, HC3H5O3, is 1.4 ✕ 10−4. (a) Suppose buffer #1 is prepared using 40.0 mL 0.1 M HC3H5O3 and 60.0 mL 0.1 M C3H5O3− to give a final volume of 100.0 mL. What is the pH of this buffer? WebAssign will check your answer for the correct number of significant figures. 4.19 Incorrect: Your answer is incorrect. (b) Suppose a buffer #2 is prepared using 60.0 mL 0.1 M HC3H5O3 and 40.0 mL 0.1 M C3H5O3−...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Calculate initial concentration of Fe+3 for tubes 1-3. Show your work. Procedure A. Determination of B...
Calculate initial concentration of Fe+3 for tubes 1-3. Show your
work.
Procedure A. Determination of B for Beer's Law 1. Using a buret, add 4.00 mL of 0.0025 M Fe(NO3)s (which is in 0.1 M HNOs) to a 100- mL volumetric flask. Add enough deionized water to bring the total volume to the mark on the neck of the flask. Stopper and shake the flask. Label this flask “Diluted Fe.” spectrophotometer tubes (cuvettes), they are too small to use at...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing and understanding what is being done. I have copied and pasted some of the text from the article. Thank you! Abstract : Obesity, high-fat diets, and subsequent type 2 diabetes (T2DM) are associated with cognitive impairment. Moreover, T2DM increases the risk of Alzheimer's disease (AD) and leads to abnormal elevation of brain beta-amyloid levels, one of the hallmarks of AD. The psychoactive alkaloid caffeine...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has its own characteristic atom in which a. the atomic mass is constant. b. the atomic number is constant. c. the mass number is constant. d. Two of the above are correct. e. All of the above are correct. 2. Which of the following is not a trace element in the human body? a. iodine b. zinc c. iron d. calcium e. fluorine 3. A...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2
For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2....the problem is that if I don't boil it for that long and (30 min) b/c of not enough time but I put the water I boiled for 1/2 hr aproximately into a NaOH bottle with a CO2 absorber and stored it there for a few days. I would assume that I would have to boil the water again...but...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Calculate initial concentration of Fe+3 for tubes 1-3. Show your
work.
Procedure A. Determination of B for Beer's Law 1. Using a buret, add 4.00 mL of 0.0025 M Fe(NO3)s (which is in 0.1 M HNOs) to a 100- mL volumetric flask. Add enough deionized water to bring the total volume to the mark on the neck of the flask. Stopper and shake the flask. Label this flask “Diluted Fe.” spectrophotometer tubes (cuvettes), they are too small to use at...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has its own characteristic atom in which a. the atomic mass is constant. b. the atomic number is constant. c. the mass number is constant. d. Two of the above are correct. e. All of the above are correct. 2. Which of the following is not a trace element in the human body? a. iodine b. zinc c. iron d. calcium e. fluorine 3. A...
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