Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the most...
Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the most accurate procedure so you end up with at least 500ul each of 5ug/ul, 0.5 ug/ul, and 0.05 ug/ul working solutions of BSA.
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Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin
(BSA), diagram the most...
How many nanomoles (nmol) of bovine serum albumin (BSA) would be present in 7.2 mL of...
How many nanomoles (nmol) of bovine serum albumin (BSA) would be present in 7.2 mL of a 1.4 mg/mL solution of BSA?
calculate the expected absorbace at 280 nm of a 1.5 mg/ml solution of bovine serum albumin...
calculate the expected absorbace at 280 nm of a 1.5 mg/ml solution
of bovine serum albumin based on the amino acid composition. all
cysteins are involved in disulfide bonds.
Calculate the expected A280 of a 1.50 mg/ml solution of bovine serum albumin, based on the amino acid composition (Table 1-1, page 28 from your manual). All the cysteines in bovine serum albumin are involved in disulfide bonds. T V W Y 10 9 0 6 12 11 0 7 6...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
BSA Standard (mg/mL) BSA Working Solution (0.5 mg/mL) ddH2O (µL) BSA Standard (µL) 1 x Bradford...
BSA Standard (mg/mL) BSA Working Solution (0.5 mg/mL) ddH2O (µL) BSA Standard (µL) 1 x Bradford Reagent (µL) OD595nm Reading 0.00 0 500 25 975 0.00 0.025 25 475 25 975 0.43 0.05 50 450 25 975 0.45 0.1 100 400 25 975 0.469 0.15 150 350 25 975 0.571 0.2 200 300 25 975 0.621 0.3 300 200 25 975 0.638 0.4 400 100 25 975 0.713 1. Construct a standard curve and plot. 2. Your unknown sample yields...
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
concentration of the stock is 39.2mg 3. (6 pts) Figure out your plan for making each...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Question: Starting with a 100,000 ug/mL manganese stock solution, write out a dilution scheme for the...
Question: Starting with a 100,000 ug/mL manganese
stock solution, write out a dilution scheme for the preparation
100mL each of 0.5, 1.0, and 2.0 mg/L calibration standards.
Note: My original scheme was to do the following,
however this is not the most efficient scheme. The amount of stock
solution that should be used is 50mL, not 100mL as shown in my
calculation.
but noo'ol = 741 Gr cealal 1 (10,000 mg/L) (V.) = (0.1L) (100 mg) V, 0.4 (100 mgle)...
A 4.0 mg/dl standard solution is needed. To prepare 100 ml of the working standard, how...
A 4.0 mg/dl standard solution is needed. To prepare 100 ml of the working standard, how much stock standard of a 1-mg/ml solution is needed? I need this one using the specific information so I understand the V1C1 = V2C2 Please explain in detail how to answer the Question. Thank you
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
calculate the expected absorbace at 280 nm of a 1.5 mg/ml solution
of bovine serum albumin based on the amino acid composition. all
cysteins are involved in disulfide bonds.
Calculate the expected A280 of a 1.50 mg/ml solution of bovine serum albumin, based on the amino acid composition (Table 1-1, page 28 from your manual). All the cysteines in bovine serum albumin are involved in disulfide bonds. T V W Y 10 9 0 6 12 11 0 7 6...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
Question: Starting with a 100,000 ug/mL manganese
stock solution, write out a dilution scheme for the preparation
100mL each of 0.5, 1.0, and 2.0 mg/L calibration standards.
Note: My original scheme was to do the following,
however this is not the most efficient scheme. The amount of stock
solution that should be used is 50mL, not 100mL as shown in my
calculation.
but noo'ol = 741 Gr cealal 1 (10,000 mg/L) (V.) = (0.1L) (100 mg) V, 0.4 (100 mgle)...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
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