BSA Standard (mg/mL) BSA Working Solution (0.5 mg/mL) ddH2O (µL) BSA Standard (µL) 1 x Bradford...

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BSA Standard (mg/mL) BSA Working Solution (0.5 mg/mL) ddH2O (µL) BSA Standard (µL) 1 x Bradford...

BSA Standard (mg/mL)	BSA Working Solution (0.5 mg/mL)	ddH2O (µL)	BSA Standard (µL)	1 x Bradford Reagent (µL)	OD595nm Reading
0.00	0	500	25	975	0.00
0.025	25	475	25	975	0.43
0.05	50	450	25	975	0.45
0.1	100	400	25	975	0.469
0.15	150	350	25	975	0.571
0.2	200	300	25	975	0.621
0.3	300	200	25	975	0.638
0.4	400	100	25	975	0.713

1. Construct a standard curve and plot.

2. Your unknown sample yields an OD595 value of 0.577. Using your standard curve, calculate the concentration of your unknown sample.

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Answer 1

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BSA Standard (mg/mL) OD595nm Reading 0.00 0.00 You can delete this point for a better correlation 0.025 0.43 Absorbance vs co

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