Question

what is the function of the akh gene? And how would you purify the AKH protein to verify the expression of the akh gene?

Answer 1

Am. - Function of Akh gene Adipo kinetic hormone mone Î Î Î Ï Akh is a peptide is involved in mobilization, of lipid a sugar from fat body of hemolymph. → It increas the body hemolymph level in. many insecto : It stimulate the heart beat in cockroach and inhibit the protein. It also increase in carbohydrate is hemolymph. - et regulate the neuropeptide signaling is the infects. - Akh gene regulate means positine. regulate the possuline. Akh gene barrically seen in or resanch on Dorsophila melanogaster" In this infect, adult carry Akhoene: It pronide energy at the time of activity and at the time of stenvation procen. akh gene control the Gst (Gretathione is the cockrooh S-transferase) actine of other insecto Ï î

Purification of Akh protein - To establish a system on the neuropeptide biosys thesis in insects. - Developed in vitro orga culture system. of corpora cardiaca (cc) of the docust. This cc consist Akh hormones AKHI & AKHI. Here tritiated amino acid added with gland and study the development of new protein ; cesiog size -exclusion and reverse phase liquid chromatography Here nee us 3-14-tryptophan on a tracer. and also raised antiserum with liquid chromatography to identify of 2 - precursor polypeptide. - size inclusion chromatography indicate the presence of c.c with 3H-tryptophan befor AKHI & II. After praction tritium - labelad pro AKH yield a palypeptide Pl&P2. Both are CPL & P2) proposed AKA pretein. into AKH genes. On that bonis Akt protein is purify. this Akh gene seen in Irget that Done sophila melanogaster.. so also named as Dorosophila gene... summay: - it crook on energy regulation in the insect. If mobilize the lipid and fat of hemolymph.