Question

6. How does Alternative splicing lead to more than one protein product from the same protein coding gene? 7. What is a noncoding RNA gene? What are some examples? 8. How do noncoding RNAs regulated gene expression? 9. If the miRNA, mir168, binds to the mRNA of the let-384 gene, would you expect gene expression of let-384 to increase or decrease?

Answer 1

0 RNA splicing is a process where mRNA exons are ligated together and introns are removed to produce a mature mRNA.SI - an alternative splicing , different combinations of exons can be ligated together to generate many mRNAs from one gene, accounting for protica diversity CS Acternative splicing regulation is important because many diseases can arise if it occurs improperly.

entron 1-3 . Pre m-RNA Exon-1 Exo-2 Excn-3 Alternate Exong Selected 3 mature mRNA. gsoform 1 Translates into Protein A Isoform 2 Translates into Protein B Fig. Alternative splicing the pre mRNA generates alternatively Spliced mRNA fooms are indicated. One contains exons. 1 & 2 and another contains Exon-2 and 3.

9 Non-coding RNA is an RNA molecule that is not translated into protein some examples of noncoding RNA are transfer RNA CERNA), ribosomal RNA (rRNA), MiRNA heSiRNA etc. C CamScanner

37 A non-coding RNA is a functional RNA molecule that is transcribed from DNA but not translated into proteens of functions to regulate the gene expression at the transcriptional and post transeriptional level. Examples oie - miRNAs, SiRNAs, PiRNAs etc. - MiRNA generally bind to a specific target messenger RNA with a complementary sequence to induce cleavage or degradation or block translation, = SiRNA s. Mediate post transcriptional gene silenceing a a result of mRNA degradation. In addition to this SiRNAS have also been shown to induce heterochromate in formation via an RNA induced transcriptional silencing complex which when bound to SiRNA promotes H3K9 methylation and chromatir condensation - PERNAC Piwi interacting RNA) is said to be involved in chromation regulation and supression of transposoon activity in germline and somatic cells. A miRNA binds to the target mRNA to induce degration or block translation - so if the miRNA mir 168 binds to the det mRNA of Let - 384 gene, It will degrade the mRNA . assogathed gene expression is expected to cease I decrease. CamScanner