Question

Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding how much protein is really present Question 4 3 pts Regulation of pattern formation during development depends upon altered gene expression. The majority of factors needed to generate these patterns regulate gene expression by: Promoting alternative splicing O Promoting protein degradation Changing phosphorylation of target proteins Altering mRNA stability Altering transcription of target genes

Answer 1

The correct answer to Q. 3- A and B

Explanation- GFP is a protein that is obtained from the jellyfish. The gene of the protein when fused with the target gene, it gets synthesized as a fusion protein. This gives certain additional benefits over the use of western blotting- those are - the GFP allows the user to track the protein in the live cells and it also does not require to lyse the cells. While, western blotting requires the cell lysis. Again GFP allows the determination of the spatial location of the protein of interest when viewed under confocal microscopy.

Why not others- C. Western blotting detects protein, not mRNA.

D.Western blotting does not amplifies the signal from the amount of the protein. The protein band in blott is directly proportional to the quantity of the protein of interest.

The correct answer to Q.4- A and E

Explanation- During development the differential expression of genes is achieved by two mechanisms- the first is Alternative RNA splicing--which employs the spliceosome machinery to recognise and cut varing introns in the mRNA. Th other is the alternative regulation of gene regulation.