Question

please answer all 3 questions asap .thanks

Question 4 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting amplifies the amount of total protein present, which prevents you from understanding how much protein is really present Western blotting detects mRNA, not protein. Question 5 3 pts What process mediates the switch in lg constant region use from constant region u to constant region ? The switch in constant region use from u to mediated by alternative splicing The switch in constant region use from u too mediated by DNA rearrangements The switch in constant region use from u too mediated by alternative promoter use The switch in constant region use from u too mediated by alternative mRNA degradation Question 6 3 pts How would inhibition of phagocytosis activity affect antigen presentation in antigen-presenting cells? Would promote antigen presentation on MHC II Would inhibit antigen presentation on MHC II Would inhibit antigen presentation on MHCI O Would promote antigen presentation on MHCI O Would have no effect on antigen presentation

Answer 1

The correct answer to q.4- A and B

Explanation- GFP gene when fused with the protein gene of interest, helps to track the protein oin live cells, both temporaly and spatially. Whereaes westren blotting requires for the cells to be lysed.

The correct answer to 6- B

Explanation- Phagocytosis is the process by which the antigen is taken inside the phagocytic cell. Only after the phagocytosis , the antigen can be processed by the exogenous pathway and then presented by the MHC II molecules. So the inhibition of the phagocytosis causes the inhibition of the MHC II expression.

Why not others-- MHC I is involved with the endogenous antigens, and that requires no phagocytosis

The correct answer to - 5-B

Explanation- There are a number of DNA segments that are alternatively rearrenaged to generate a diverse repertoire of antibody and this same mechanismfollows for the class switching purpose