Question

1 and 2 please

Question 1 3 pts Regulation of pattern formation during development depends upon altered gene expression. The majority of factors needed to generate these patterns regulate gene expression by: Changing phosphorylation of target proteins Altering mRNA stability Promoting protein degradation Altering transcription of target genes Promoting alternative splicing Question 2 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting amplifies the amount of total protein present, which prevents you from understanding how much protein is really present Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting detects mRNA, not protein.

Answer 1

1. Answer: Option D is correct
Explanation:
Pattern formation during development establishes the overall body plan of an organism.
Many pattern formation genes are transcription factors. SO, they establish the pattern formation by activating or repressing transcription.
All the other cases are also present but to a smaller extent.

2. Answer: Options A, B, and C are correct
Explanation:
Western blotting is a disruptive technique i.e. the cell lysate is used for the protein quantification using an antibody.
It is not an absolute quantification method.
It does not provide spatial and temporal expression patterns.