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QUESTION 21 Consider the following graph and answer questions 21 through 24 below: 10 Calen 02 m Wய பாய) 5 1/[S] (ma!) 24. Wh

QUESTION 22 What is the approximate Vmax for [U] = 4 mM? OA 0.25 PM/min, OB. 0.50 LM/min, 02.00 LM/min, OD.3.00 uM/min, E. 4.

QUESTION 24 What is the ki or K? O A. 0.25 mm OB. 0.5 mm OC. 1 mM OD.2 mm OE. 4 mm OF. 8 MM

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Answer #1

21. This image of 'Lineweaver Burk plot' is mentioning a reciprocal plot of V and [S] i.e. 1/V and 1/[S].

And, according to Lineweaver Burk plot,

1/V= Km/Vmax.1/[S] +1/Vmax

Following this equation,

the point where the straight line touches the X axis is negative value of 1/Km (-1/Km).

24. The approximate Km for the control (No [l]) is

-1/Km= -5 mM Or, Km=2mM

22. Approximate Vmax for 4mM Km value will be

1/Vmax= Y intercept which is near to 1/V=3mM/min or V=1/4 mM/min i.e. 0.25mM/min

24. The KI values are 0.5mM

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