Homework Answers
C) you analyze whether there has been alternative splicing by examining amino acids of very similar receptor gene.
Even after a gene has been transcribed, gene expression can still be regulated at various stages. Some transcripts can undergo alternative splicing, making different mRNAs and proteins from the same RNA transcript.
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on 6 of 20 0 points Save A You are trying to understand how the expression...
molecular biology Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory r...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
please answer all 6 questions Question 27 3 pts TRBP is a protein important for the...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
Name 2ors L! You are inte finding protein are interested in understanding how Top5 expression is...
Name 2ors L! You are inte finding protein are interested in understanding how Top5 expression is regulated. Top5 is a microtubule protein. You study a cell line where Top5 expression is controlled by a signal, Ligl. When hinds to and activates the membrane bound recentor it activates the Ras protein, which activates atein kinase called PKF, which then phosphorylates Grp4 on T224. Grp4 is a gene regulatory Wotein that can only enter the nucleus when phosphorylated: Thos is not expressed...
Yet, all the cells in your body contain the same genes (and same alleles). The difference...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
Name 2ors L! You are inte finding protein are interested in understanding how Top5 expression is regulated. Top5 is a microtubule protein. You study a cell line where Top5 expression is controlled by a signal, Ligl. When hinds to and activates the membrane bound recentor it activates the Ras protein, which activates atein kinase called PKF, which then phosphorylates Grp4 on T224. Grp4 is a gene regulatory Wotein that can only enter the nucleus when phosphorylated: Thos is not expressed...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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